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Susceptibility of an Insect Leptomonas and Crithidia fasciculata to Several Established Antitrypanosomatid Agents

1974; American Society for Microbiology; Volume: 6; Issue: 6 Linguagem: Inglês

10.1128/aac.6.6.785

1098-6596

Cyrus J. Bacchi, C. Lambros, Burt Goldberg, S. H. Hutner, Gabriel Domingos Carvalho,

Insect-Plant Interactions and Control

Growth inhibition of the lower trypanosomatids Crithidia fasciculata and a Leptomonas from a hemipteron by several established trypanocides and leishmanicides were compared in four complex and one defined media. The Leptomonas was more susceptible than C. fasciculata in all media, especially to phenanthridines (ethidium, prothidium, isometamidium) and diamidines (pentamidine, diminazene diaceturate [Berenil], hydroxystilbamidine, stilbamidine); concentrations of these drugs required for 50% inhibition of the Leptomonas were <5 μg/ml. In contrast, C. fasciculata was uninhibited by <20 μg of diamidines per ml and was three- to sixfold less susceptible than the Leptomonas to isometamidium and prothidium. Both trypanosomatids were susceptible to nucleoside antibiotics, e.g., nucleocidin. Neither was inhibited by suramin, melarsen, melarsen oxide, or tryparsamide. The Leptomonas was more susceptible to standard trypanocides than five other insect trypanosomatids in a complex medium; it was the only one inhibited by <20 μg of stilbamidine and hydroxystilbamidine per ml.