The use of an automatic on-line system for monitoring penicillin cultivation in a bubble-colum loop reactor
1986; Elsevier BV; Volume: 190; Linguagem: Inglês
10.1016/s0003-2670(00)82881-0
ISSN1873-4324
AutoresJ. Niehoff, J. Möller, R. Hiddessen, K. Schügerl,
Tópico(s)Analytical Chemistry and Sensors
ResumoPenicillium chrysogenum is cultivated by a fed-batch mode to produce penicillin V. During fermentation, the concentrations of the medium components must be held at predetermined levels, which will change during fermentation, e.g., in the growth phase the concentration of the carbon sources and the nitrogen sources (urea and ammonium) must be high enough to maximize biomass production, whereas in the production stage these sources should be limited. To achieve optimal substrate concentrations, continuous measurement of various components in the fermentation broth is necessary. This is done by using a sterilizable ultrafiltation sampling probe and an air-segmented automatic flow analysis system to determine reducing sugars, dissolved organic carbon, ammonium, urea, sulfate, phosphate and penicillin V concentrations; spectrophotometric and gas-sensing electrodes are used in order to guarantee dependable results throughout the 290-h fermentation process, the analysis system is automatically cleaned and calibrated, and blanks are determined. The results are stored and evaluated by computer.
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