Standard biobanking conditions prevent evaporation of body fluid samples
2015; Elsevier BV; Volume: 442; Linguagem: Inglês
10.1016/j.cca.2015.01.036
ISSN1873-3492
AutoresEline A.J. Willemse, Marleen J.A. Koel‐Simmelink, Sisi Durieux‐Lu, Wiesje M. van der Flier, Charlotte E. Teunissen,
Tópico(s)Biosimilars and Bioanalytical Methods
ResumoPre-analytical variation in biobanking procedures, e.g., long-term storage, could confound biomarker outcomes. We investigated evaporation in various body fluids at different storage temperatures and storage durations. Biobank sample tubes (Sarstedt 72.694.007) filled with water in different volumes (50, 100, 250, 500, 750, 1000, 1250, 1500 μl) were stored at different temperatures (− 80 °C, − 20 °C, 4 °C, room temperature (RT)) for 4.5 years and weighed at regular intervals. Next, saliva, serum, plasma, and CSF were stored in different volumes (50, 250, 500, 1000 μl) at different temperatures (− 80 °C, − 20 °C, 4 °C, RT) for 2 years. An extra set of CSF was stored in tubes with safe-lock cap (Eppendorf 0030 120.086) instead of a screw cap with o-ring. No evaporation of water stored in biobanking tubes at − 80 °C or − 20 °C occurred over 4.5 years. Storage of saliva, serum, plasma, and CSF at − 80 °C or − 20 °C, monitored over 2 years, protected these samples from evaporation too. At 4 °C, evaporation was minor, approximately 1.5% (50 μl) or 0% (1 ml) yearly, where at RT it ranged from 38% (50 μl) to 2% (1 ml). Differences were observed neither between different body fluids, nor between tube caps. Our data provide support for long-term biobanking conform current biobanking guidelines, encouraging retrospective use of clinical cohorts.
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