Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli
1987; Elsevier BV; Volume: 58; Issue: 2-3 Linguagem: Inglês
10.1016/0378-1119(87)90383-0
ISSN1879-0038
AutoresHelen Shuttleworth, Clive J. Duggleby, Susan A. Jones, Tony Atkinson, Nigel P. Minton,
Tópico(s)Bacteriophages and microbial interactions
ResumoNucleotide sequence analysis of the gene (spa) for staphylococcal protein A (SPA) from Staphylococcus aureus strain Cowan l (NCTC8530) shows that the sequence differs from previously reported SPA nucleotide sequences, especially in the number of repeat units in the cell-wall-binding region of the gene. Dot matrix comparison with streptococcal protein G and the macrophage receptor for the constant fragment (Fc) of immunoglobulins shows a limited but significant homology. The homology to the latter probably identifies the Fc-binding region in the immunoglobulin-binding domains of SPA. Enhanced production of SPA in Escherichia coli was achieved using the lac promoter immediately upstream from the spa gene.
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