First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Potato and Other Vegetables in Poland
2015; American Phytopathological Society; Volume: 99; Issue: 9 Linguagem: Inglês
10.1094/pdis-02-15-0180-pdn
ISSN1943-7692
AutoresMałgorzata Waleron, Krzysztof Waleron, Ewa Łojkowska,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 99, No. 9First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Potato and Other Vegetables in Poland PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Potato and Other Vegetables in PolandM. Waleron, K. Waleron, and E. LojkowskaM. Waleron, K. Waleron, and E. LojkowskaAffiliationsAuthors and Affiliations M. Waleron , Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-822 Gdansk K. Waleron , Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland E. Lojkowska , Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-822 Gdansk. Published Online:24 Jul 2015https://doi.org/10.1094/PDIS-02-15-0180-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Blackleg and soft rot diseases cause severe economic losses in potato (Solanum tuberosum) production all over the world. Bacteria from the genera Pectobacterium and Dickeya have been isolated from blackleg and soft rot potato (Toth et al. 2011). Among bacteria isolated from symptomatic plants in Poland, Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum dominate (Waleron et al. 2002). Recently, strains of Pectobacterium carotovorum subsp. brasiliense were isolated from plants collected in different countries (De Boer et al. 2012, Nabhan et al. 2012). In light of this fact, we investigated isolates collected from plants with soft rot symptoms since 1995 on the territory of Poland in search of P. c. subsp. brasiliense. All strains were stored in 40% glycerol at –80°C. Among 160 strains identified earlier as P. carotovorum (Waleron et al. 2002), three strains isolated from potato (IFB5348, IFB5368, and IFB5331), and one each from cabbage (IFB5260), sugar beet (IFB5258), and zucchini (IFB5259), produced a 322-bp PCR product with primers (BR1f/L1R) specific for P. carotovorum subsp. brasiliense (Duarte et al. 2004). All isolates were gram-negative, facultative anaerobes exhibiting pectinolytic activity, and were negative for oxidase, urease, indol production, gelatin liquefaction, and acid production from D-arabitol, dulicitol, and sorbitol. All strains were unable to utilize malonate and citrate. However, they were catalase positive, produced acid from lactose, rhamnose, and trehalose, and they grew at both 28°C and 37°C. All strains except for IFB5368 were resistant to 6% NaCl. Strain IFB5348 differed from the remaining strains; it neither produced reducing substances from sucrose, utilized α-methyl-D-glucoside, nor produced acid from maltose. All strains isolated from potato caused soft rot symptoms on potato tuber tissues. The mean diameter of rotting potato tubers tissue (15.73 mm) was measured after incubation in 28°C and 95% relative humidity for 72 h. Surface-sterilized potato tubers were inoculated by inserting 25 µl of bacterial suspension (2.0 × 107 CFU/ml). This experiment consisted of three replicate tubers per strain, and it was performed three times. As a negative control, sterile water was used. PCR with Br1f/L1r primers confirmed that the bacteria isolated from rotted tissue were P. carotovorum subsp. brasiliense. Analysis of the 16S rRNA gene sequences (KP762580 to KP76258) of six aforementioned strains indicated that all of them (sharing identity 99.2 to 99.6% between each other) exhibited 99 to 100% identity with P. carotovorum subsp. brasiliense, strains PBR1692 and LMG21371 (GenBank Accession Nos. ABVX00000000 and JQOE00000000). Further analysis was performed on concatenated sequences of recA and rpoS genes (KP762574-KP76279, KP762586-KP76291). The consensus tree constructed using Maximum Likelihood method clustered strains IFB5258-60, IFB5348, IFB5368, and IFB5331 with P. carotovorum subsp. brasiliense strains available in GenBank. To our knowledge, this is the first report of P. carotovorum subsp. brasiliense causing soft rot on potato, sugar beet, cabbage, and zucchini in Poland. Moreover, we can state that this subspecies was present in Poland as early as 1996.References:Toth, I. K., et al. 2011. 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Microbiol. 113:904. https://doi.org/10.1111/j.1365-2672.2012.05383.x Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 9 September 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 27 Aug 2015Published: 24 Jul 2015First Look: 20 Apr 2015Accepted: 16 Apr 2015 Pages: 1271-1271 Information© 2015 The American Phytopathological SocietyCited byOccurrence, identification, and host range of Pectobacterium brasiliense causing soft rot on seed potato tubers in Turkey20 October 2022 | Journal of Plant Diseases and Protection, Vol. 66Genetic Diversity of Pectobacterium spp. on Potato in Serbia15 September 2022 | Microorganisms, Vol. 10, No. 9Inactivation of Pectobacterium carotovorum subsp. 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