Application of spheroid culture to human hepatocytes and maintenance of their differentiation
1994; Wiley; Volume: 81; Issue: 1 Linguagem: Inglês
10.1016/0248-4900(94)90058-2
ISSN1768-322X
AutoresJian Zhong Tong, Sophie Sarrazin, Doris Cassio, Frédéric Gauthier, Fernando Álvarez,
Tópico(s)3D Printing in Biomedical Research
ResumoSummary— Human hepatocytes cultured with a hormonally defined medium on non‐adherent poly‐(2‐hydroxyethyl methacrylate) coated surface were able to form spheroids. The maintenance of liver‐specific functions was assessed by following secretion of albumin, transferrin and α‐antitrypsin that were still detectable after 4 months of spheroidal culture. Moreover, cytochrome P‐450 IA was induced by methylcholanthrene for up to 2 weeks. This cell system is very promising for long‐term in vitro studies of human hepatocyte functions.
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