Cybrid/hybrid plants regenerated from somatic fusions between male sterile Satsuma mandarin and seedy tangelos
2009; Elsevier BV; Volume: 122; Issue: 2 Linguagem: Inglês
10.1016/j.scienta.2009.05.017
ISSN1879-1018
AutoresXiaodong Cai, Jing Fu, Chunli Chen, Wen‐Wu Guo,
Tópico(s)Plant Reproductive Biology
ResumoSomatic hybridization provides an alternative for transferring mitochondria-encoded cytoplasmic male sterility (CMS). Herein, symmetric protoplast electrofusion was conducted between embryogenic callus protoplasts of Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a CMS cultivar, and mesophyll-derived protoplasts of seedy 'Page' tangelo [C. reticulata Blanco × (C. reticulata Blanco × C. paradisi Macf.)] or 'Nova' tangelo [C. reticulata Blanco × (C. reticulata Blanco × C. paradisi Macf.)], to transfer CMS trait. Flow cytometry analysis showed that 14 plants recovered from G1 + 'Page' tangelo that displayed typical morphological character of 'Page' were diploid, and 6 plants regenerated from G1 + 'Nova' tangelo were tetraploid. Genetic compositions of regenerated plants from the two fusions were determined by SSR, CAPS and chloroplast-SSR analysis. Cybrid nature of diploids from G1 + 'Page' tangelo with nuclear DNA from 'Page', mitochondrial DNA (mtDNA) from the G1 and chloroplast DNA (cpDNA) derived from either parent was confirmed. Tetraploid plants from G1 + 'Nova' tangelo were identified as somatic hybrids with random cpDNA inheritance. The regenerated cybrid and hybrid plants hold great potential for Citrus seedless breeding at diploid or triploid levels.
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