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Ventral Approach to the Rat Middle Ear for Otologic Research

2001; Wiley; Volume: 124; Issue: 5 Linguagem: Inglês

10.1067/mhn.2001.115370

1097-6817

Mayte Pinilla, Rafael Ramírez‐Camacho, Eduardo Jorge, Almudena Trinidad, José Vergara,

Biochemical Analysis and Sensing Techniques

OBJECTIVE To describe a simple approach to the rat middle ear because there are few clear descriptions of this technique with minimal morbidity and mortality. STUDY DESIGN AND SETTING Six male Long‐Evans rats were used in this study. We designed a cervical approach to the middle ear with the purpose of creating an experimental model of ossiculoplasty that permits the postoperative survival of the animal. RESULTS It was possible in all cases to visualize the majority of structures of the tympanic cavity: promontory, round window, stapedial artery, stapes, incus, and tympanic membrane. There were no cases of postoperative infection or facial paralysis. CONCLUSIONS AND SIGNIFICANCE The ventral approach to the rat middle ear is a rapid and simple technique that makes the rat the animal of choice for many otologic investigations.