Artigo Revisado por pares

Fourier Transform Infrared Spectroscopy as a Method for Monitoring the Molecular Dynamics of Lymphocyte Activation

2000; SAGE Publishing; Volume: 54; Issue: 3 Linguagem: Inglês

10.1366/0003702001949627

ISSN

1943-3530

Autores

Bayden R. Wood, Brian D. Tait, Don McNaughton,

Tópico(s)

Animal Virus Infections Studies

Resumo

In this paper we report the application of Fourier transform infrared (FT-IR) microspectroscopy to monitor the molecular dynamics of lymphocyte activation. Infrared spectra of lymphocytes stimulated with the mitogen phytohaemagglutinin-L show spectral features 15 min after initial stimulation that are not apparent in resting lymphocytes. By analyzing the second-order derivatives of the raw spectra and applying principal components analysis (PCA), we conclude that the major spectral changes observed in the first hour result from an increase in overall RNA synthesis. Bands characteristic of RNA at 1244, 1080, 1050, 970, 1160, and 1120 cm −1 appear progressively more intense over time in the spectra of activated lymphocytes. The magnitude of these changes increases over time as the cell differentiates into a blast cell. The sensitivity of infrared spectroscopy to RNA moieties and the rapidity of the technique suggest a possible future role for FT-IR spectroscopy in histocompatibility testing.

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