Methylsulphone metabolites of m -dichlorobenzene as ligands for a 2u - g lobulin in rat kidney and urine
1990; Taylor & Francis; Volume: 20; Issue: 1 Linguagem: Inglês
10.3109/00498259009046808
ISSN1366-5928
AutoresG. L. Larsen, J. E. Bakke, Janice K. Huwe,
Tópico(s)Metabolism and Genetic Disorders
Resumo1. Kidneys from rats given oral doses of either 3,5- or 2,4- dichlorophenyl[14C]methylsulphide contained the corresponding sulphones that were associated with a protein which has been isolated and characterized by SDS-polyacrylamide gel electrophoresis and immunoblot analysis to be alpha 2u-globulin. 2. The same methylsulphone-alpha 2u-globulin complex was isolated from the urine from rats doses with 3,5-dichlorophenyl[14C]methylsulphide (0.3-1.0% of dose, 8.5% of 14C in urine. 3. The stoichiometry of binding of the isolated methylsulphone-alpha 2u-globulin complex was shown to be 0.54-0.78 nmol/nmol protein. This binding was not covalent.
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