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The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor with a Novel Conserved Auxiliary DNA-Binding Motif

2008; American Chemical Society; Volume: 47; Issue: 44 Linguagem: Inglês

10.1021/bi800961d

1943-295X

Ricardo Medina, Timothy Buck, Sayyed K. Zaidi, Angela Miele-Chamberland, Jane B. Lian, Janet L. Stein, André J. van Wijnen, Gary S. Stein,

RNA modifications and cancer

Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of human histone gene expression at the G1−S phase transition plays a critical role. The zinc finger HiNF-P and coactivator p220NPAT proteins are key regulators of histone gene expression. Here, we describe a novel HiNF-P-specific conserved region (PSCR) located within the C-terminus that is present in HiNF-P homologues of all metazoan species that have been examined. The PSCR motif is required for activation of histone H4 gene transcription and contributes to DNA binding of HiNF-P. Thus, the PSCR module represents an auxiliary DNA-binding determinant that plays a critical role in mediating histone gene expression during the cell cycle and defines HiNF-P as a unique cell cycle regulatory member of the zinc finger transcription factor family.