Probing the secondary quinone (Q B ) environment in photosynthetic bacterial reaction centers by light‐induced FTIR difference spectroscopy
1991; Wiley; Volume: 288; Issue: 1-2 Linguagem: Inglês
10.1016/0014-5793(91)81014-y
ISSN1873-3468
AutoresJ. Breton, Catherine Berthomieu, D. L. Thibodeau, E. Nabedryk,
Tópico(s)Spectroscopy and Quantum Chemical Studies
ResumoThe photoreduction of the secondary electron acceptor, QB, has been characterized by light-induced Fourier transform infrared difference spectroscopy of Rb. sphaeroides and Rp. viridis reaction centers. The reaction centers were supplemented with ubiquinone (UQ10 or UQ0). The QB- state was generated either by continuous illumination at very low intensity or by single flash in the presence of redox compounds which rapidly reduce the photooxidized primary electron donor P+. This approach yields spectra free from P and P+ contributions making possible the study of the microenvironment of QB and QB-. Assignments are proposed for the C...O vibration of QB- and tentatively for the C = O and C = C vibrations of QB.
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