Determination of aflatoxin Q1 in urine by automated immunoaffinity column clean-up and liquid chromatography
1994; Elsevier BV; Volume: 656; Issue: 2 Linguagem: Inglês
10.1016/0378-4347(94)00123-5
ISSN1872-812X
AutoresAnders Kussak, Barbro Andersson, Kurt Andersson,
Tópico(s)Indoor Air Quality and Microbial Exposure
ResumoA liquid chromatographic system with an automated clean-up procedure for aflatoxin Q1 in human urine is described. The samples were cleaned up by using immunoaffinity columns originally designed for aflatoxin M1. The chromatographic system was a C18 column with an acidic mobile phase of acetonitrile-water containing potassium bromide. Fluorescence detection (365/440 nm) of aflatoxin Q1 was enhanced by addition of bromine, using post-column derivatization, which was studied by factorial designs. Average recovery of aflatoxin Q1 in spiked 10-ml urine samples was 88% (R.S.D. = 6.4%) at a level of 50 pg/ml. The determination limit was 49.5 pg/ml urine.
Referência(s)