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Complement activation by IgG immobilized on methylated silicon

1996; Wiley; Volume: 31; Issue: 3 Linguagem: Inglês

10.1002/(sici)1097-4636(199607)31

1097-4636

Pentti Tengvall, Agneta Askendal, I. Lundstr�m,

Monoclonal and Polyclonal Antibodies Research

Journal of Biomedical Materials ResearchVolume 31, Issue 3 p. 305-312 Complement activation by IgG immobilized on methylated silicon Pentti Tengvall, Corresponding Author Pentti Tengvall Laboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenLaboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenSearch for more papers by this authorAgneta Askendal, Agneta Askendal Laboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenSearch for more papers by this authorIngemar Lundström, Ingemar Lundström Laboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenSearch for more papers by this author Pentti Tengvall, Corresponding Author Pentti Tengvall Laboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenLaboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenSearch for more papers by this authorAgneta Askendal, Agneta Askendal Laboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenSearch for more papers by this authorIngemar Lundström, Ingemar Lundström Laboratory of Applied Physics, Linköping University S-581 83 Linköping, SwedenSearch for more papers by this author First published: July 1996 https://doi.org/10.1002/(SICI)1097-4636(199607)31:3 3.0.CO;2-QCitations: 34AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Activation of the complement system by immobilized IgG on methylated silicon was studied by ellipsometry/antibody-, ELISA-, and RIA techniques after exposure to human serum at 37°C for up to 1 h. The IgG-covered surfaces rapidly activated the complement system and the combined results suggest an initial classical pathway activation. Complement factor 1q (C1q) and IgG were antibody-detectable on the surfaces for serum incubations up to 5 min but not thereafter. Anti-C3c and anti-properdin bound to the surfaces at all serum incubation times. Experiments with 125I-IgG preadsorbed to surfaces, or added to normal-, EGTA-, and EDTA-sera, showed that IgG was not displaced from the protein film by serum. © 1996 John Wiley & Sons, Inc. Citing Literature Volume31, Issue3July 1996Pages 305-312 RelatedInformation