Determination of human urinary hyaluronic acid, chondroitin sulphate and dermatan sulphate as their unsaturated disaccharides by high-performance liquid chromatography
1991; Elsevier BV; Volume: 565; Issue: 1-2 Linguagem: Inglês
10.1016/0378-4347(91)80378-p
ISSN1872-812X
AutoresHidenao Toyoda, Kazuhiro Motoki, Masahiko Tanikawa, Kazufusa Shinomiya, Hiroshi Akiyama, Toshio Imanari,
Tópico(s)Seaweed-derived Bioactive Compounds
ResumoA method for the determination of hyaluronic acid (HA), chondroitin sulphate (CS) and dermatan sulphate (DS) was developed. HA, CS and DS were converted to the corresponding unsaturated disaccharides by digestion with chondroitinase ABC and/or chondroitinase AC-II and determined by high-performance liquid chromatography with fluorimetric detection using 2-cyanoacetamide as a post-column derivatization reagent. The calibration graphs for the unsaturated disaccharides were linear over the range 2 ng - 2 micrograms for each unsaturated disaccharide. This method was applied to the analysis of normal human urine.
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