A large plasmid from Halobacterium halobium carrying genetic information for gas vacuole formation
1979; Elsevier BV; Volume: 2; Issue: 3 Linguagem: Inglês
10.1016/0147-619x(79)90021-0
ISSN1095-9890
AutoresGottfried Weidinger, Günther Klotz, Werner Goebel,
Tópico(s)Protist diversity and phylogeny
ResumoA large plasmid with a molecular weight of 100 × 106 has been found in Halobacterium halobium which is indistinguishable from the previously described “satellite DNA.” In this halophilic bacterium characteristic properties such as the biosyntheses of gas vacuoles, purple membrane, and ruberin are spontaneously lost at high frequencies. These phenotypic alterations are accompanied by a change in the nucleotide sequence of the plasmid DNA. In plasmids of vacuole-deficient mutants two distinct PstI fragments in the restriction pattern are altered probably by an insertion of 3600 base pairs into the DNA. In revenants which form gas vacuoles the original sequence of the plasmid DNA is restored. This indicates that the presence of the plasmid is related to the gas vacuole formation.
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