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R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)

1999; Brazilian Society of Genetics; Volume: 22; Issue: 2 Linguagem: Inglês

10.1590/s1415-47571999000200011

1678-4685

Edson Luis Maistro, Fausto Foresti, Cláudio Oliveira,

CRISPR and Genetic Engineering

The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.