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Dose-response effects of the antioxidant α-lipoic acid in the liver and brain of pompano Trachinotus marginatus (Pisces, Carangidae)
2013; Wiley; Volume: 29; Issue: 5 Linguagem: Inglês
10.1111/jai.12137
ISSN1439-0426
AutoresMateus Tavares Kütter, José María Monserrat, Renato Adriano dos Santos, Marcelo Borges Tesser,
Tópico(s)Coenzyme Q10 studies and effects
ResumoJournal of Applied IchthyologyVolume 29, Issue 5 p. 1123-1128 Original Article Dose-response effects of the antioxidant α-lipoic acid in the liver and brain of pompano Trachinotus marginatus (Pisces, Carangidae) M. T. Kütter, M. T. Kütter Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Laboratório de Nutrição de Organismos Aquáticos, Instituto de Oceanografia (IO-FURG), FURG, BrasilSearch for more papers by this authorJ. M. Monserrat, J. M. Monserrat Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Instituto de Ciências Biológicas (ICB) FURG, BrasilSearch for more papers by this authorR. A. Santos, R. A. Santos Programa de Pós-Graduação em Ciências Fisiológicas–Fisiologia Animal Comparada, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Instituto de Ciências Biológicas (ICB) FURG, BrasilSearch for more papers by this authorM. B. Tesser, Corresponding Author M. B. Tesser Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Laboratório de Nutrição de Organismos Aquáticos, Instituto de Oceanografia (IO-FURG), FURG, Brasil Author's address: M. B. Tesser, Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Av. Itália km 8, Rio Grande, RS, Cx. P. 474, CEP 96.200-970, Brasil. E-mail: [email protected]Search for more papers by this author M. T. Kütter, M. T. Kütter Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Laboratório de Nutrição de Organismos Aquáticos, Instituto de Oceanografia (IO-FURG), FURG, BrasilSearch for more papers by this authorJ. M. Monserrat, J. M. Monserrat Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Instituto de Ciências Biológicas (ICB) FURG, BrasilSearch for more papers by this authorR. A. Santos, R. A. Santos Programa de Pós-Graduação em Ciências Fisiológicas–Fisiologia Animal Comparada, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Instituto de Ciências Biológicas (ICB) FURG, BrasilSearch for more papers by this authorM. B. Tesser, Corresponding Author M. B. Tesser Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Rio Grande, RS, Brasil Laboratório de Nutrição de Organismos Aquáticos, Instituto de Oceanografia (IO-FURG), FURG, Brasil Author's address: M. B. Tesser, Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande- FURG, Av. Itália km 8, Rio Grande, RS, Cx. P. 474, CEP 96.200-970, Brasil. E-mail: [email protected]Search for more papers by this author First published: 20 January 2013 https://doi.org/10.1111/jai.12137Citations: 10Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary This study evaluated the effect of different doses of the antioxidant α-lipoic acid (LA) administered by intraperitoneal injection on the detoxifying capacity (activity of glutathione-S-transferase, GST) and oxidative damage (lipids and proteins) in the pompano, Trachinotus marginatus. The plasma glucose levels showed that there were no differences between the treatments (P > 0.05). In the brain, GST activity was significantly higher (P < 0.05) in fish injected with 40 mg LA kg−1 when compared with the control group. In the muscle, GST activity was not influenced by LA treatment (P > 0.05). In the liver, fish injected with 20 mg LA kg−1 showed higher GST activity than the control group (P < 0.05); however, higher doses (40 and 60 mg LA kg−1) led to a reduction of GST activity in the liver, which was comparable to that observed in the control group (P > 0.05). The two highest LA doses (40 and 60 mg kg−1) had opposite effects, depending on the tissue examined: LA was an antioxidant in the brain, reducing lipid peroxidation (P < 0.05), and a pro-oxidant in the liver, augmenting oxidative lipid damage (P < 0.05). The latter effect was accompanied by an increase in the free iron concentration in the liver at higher LA doses. These results indicate the need to thoroughly evaluate the antioxidant effects on aquatic organisms, since at some doses and/or in some organs their beneficial effects can be lost. References Amado, L. L.; Garcia, M. L.; Pereira, T. C. B.; Yunes, J. S.; Bogo, M. R.; Monserrat, J. M., 2011: Chemoprotection of lipoic acid against microcystin-induced toxicosis in common carp (Cyprinus carpio, Cyprinidae). Comp. Biochem. Physiol. 154(C), 146– 153. Arivazhagan, P.; Thilakavathy, T.; Ramanathan, K.; Kumaran, S.; Panneerselvam, C., 2002: Effect of DL-α-lipoic acid on the status of lipid peroxidation and protein oxidation in various brain regions of aged rats. J. Nutrit. Biochem. 13, 619– 624. Atukeren, P.; Aydin, S.; Uslu, E.; Gumustas, K. M.; Çakatay, U., 2010: Redox homeostasis of albumin in relation to alfa-lipoic acid and dihydrolipoic acid. Oxidativy Med. Cell. Longev. 3, 206– 213. Bahtt, K.; Flora, S. J. S., 2009: Oral co-administration of α-lipoic acid, quercetin and captopril prevents gallium arsenide toxicity in rats. Environ. Toxicol. Pharm. 28, 140– 146. Bergamini, C. M.; Gambetti, S.; Dondi, A.; Cervellati, C., 2004: Oxygen, reactive oxygen species and tissue damage. Curr. Pharm. Des. 10, 1611– 1626. Biewenga, G. P. H.; Haenen, G. R. M. M.; Bast, A., 1997: The pharmacology of the antioxidant lipoic acid. Gen. Pharmacol. 29, 315– 331. Blanchette, B.; Feng, X.; Singh, B. R., 2007: Marine glutathione S-transferase. Mar. Biotechnol. 9, 513– 542. Block, G., 1991: Vitamin C and cancer prevention: the epidemiologic evidence. Amer. J. Clin. Nutr. 53, 270S– 282S. Butler, J. A.; Hagen, T. M.; Moreau, R., 2009: Lipoic acid improves hypertriglyceridemia by stimulating triacylglycerol clearance and down regulating liver triacylglycerol secretion. Arch. Biochem. Biophys. 485, 63– 71. Çakatay, U.; Kayali, R.; Sivas, A.; Tekeli, F., 2005: Prooxidant activities of alfa-lipoic acid on oxidative protein damage in the aging rat heart muscle. Arch. Geront. Geriatr. 40, 231– 240. Chng, H. T.; New, L. S.; Neo, A. H.; Goh, C. W.; Browne, E. R.; Chan, E. C. Y., 2009: Distribution study of orally administered lipoic acid in rat brain tissues. Brain Res. 1251, 80– 86. Christensen, K.., 1983: Vitamins. pp. 215– 272. In: Dynamic biochemistry of animal production. Ed: Riis. P. M., Elsevier Science, Amsterdam, 501pp. ISBN0444420525. Costa, L. F.; Miranda-Filho, K. C.; Severo, M. P.; Sampaio, L. A., 2008: Tolerance of juvenile pompano Trachinotus marginatus to acute ammonia and nitrite exposure at different salinity levels. Aquaculture 285, 270– 272. Cui, J.; Guowei, L.; Yang, R.; Shi, Y., 2009: Lipoic acid attenuates high fat diet-induced chronic oxidative stress and immunosuppression in mice jejunum: a microarray analysis. Cell. Immunol. 260, 44– 50. Cunha, V. L.; Rodrigues, R. V.; Okamoto, M. H.; Sampaio, L. A., 2009: Consumo de oxigênio pós-pandrial de juvenis do pampo Trachinotus marginatus. Ciênc. Rural 39, 1257– 1259. Diaz-Cruz, A.; Serret, M.; Ramirez, G.; Ávila, E.; Guinzberg, R.; Pina, E., 2003: Prophylactic action of lipoic acid on oxidative stress and growth performance in broilers at risk of developing ascites syndrome. Avian Pathol. 32, 645– 653. Ferreira, P. M. P.; Militão, G. C. G.; Freitas, R. M., 2009: Lipoic acid effects on lipid peroxidation level, superoxide dismutase activity and monoamines concentration in rat hippocampus. Neurosci. Lett. 464, 131– 134. Freitas, R. M., 2009: The evaluation of effects of lipoic acid on the lipid peroxidation, nitrite formation and antioxidant enzymes in the hippocampus of rats after pilocarpine-induced seizures. Neurosci. Lett. 455, 140– 144. Goralska, M.; Dackor, R.; Holley, B.; McGahan, M. C., 2003: Alpha lipoic acid changes iron uptake and storage in lens epithelial cells. Exp. Eye Res. 76, 241– 248. Habig, W. H.; Jakoby, W. B., 1981: Assays for differentiation of glutathione S-transferases. Meth. Enzymol. 77, 398– 405. Habig, W. H.; Pabst, M. J.; Jakoby, W. B., 1974: Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130– 7139. Hamre, K.; Lie, O., 1995: α-Tocopherol levels in different organs of Atlantic salmon (Salmo salar L.): effect of smoltification, dietary levels of n-3 polyunsaturated fatty acids and vitamin E. Comp. Biochem. Physiol. 4, 547– 554. Haramaki, N.; Han, D.; Handelman, G. J.; Tritschler, H. J.; Packer, L., 1997: Cytosolic and mitochondrial systems for NADH- and NADPH dependent reduction of alpha-lipoic acid. Free Radic. Biol. Med. 22, 535– 542. Henriksen, E. J., 2006: Exercise training and the antioxidant a-lipoic acid in the treatment of insulin resistance and type 2 diabetes. Free Radic. Biol. Med. 40, 3– 12. Hill, A. S.; Werner, J. A.; Rogers, Q. R.; O'Neil, S. L.; Christopher, M. M., 2004: Lipoic acid is 10 times more toxic in cats than reported in humans, dogs or rats. J. Animal Physiol. Animal Nutr. 88, 150– 156. Jiang, J.; Zheng, T.; Zhou, X.; Liu, Y.; Feng, L., 2009: Influence of glutamine and vitamin E on growth and antioxidant capacity of fish enterocytes. Aquacult. Nutrit. 15, 409– 414. Monserrat, J. M.; Ventura, J. L.; Ferreira, R.; Acosta, D.; Garcia, M. L.; Ramos, P. B.; Moraes, T. B.; Dos-Santos, L. C.; Amado, L. L., 2008: Modulation of antioxidant and detoxification responses mediated by lipoic acid in the fish Corydoras paleatus (Callychthyidae). Comp. Biochem. Physiol. 148(C), 287– 292. Navari-Izzo, F.; Quartacci, M.; Sgherri, C., 2002: Lipoic acid: a unique antioxidant in the detoxification of activated oxygen species. Plant Physiol. Biochem. 40, 463– 470. Oakes, K. D.; Van der Kraak, G. J., 2003: Utility of the TBARS assay in detecting oxidative stress in white sucker (Catostomus commersoni) populations exposed to pulp mill effluent. Aquat. Toxicol. 63, 447– 463. Okamoto, M. H.; Tesser, M. B.; Louzada, L. R.; Santos, R. A.; Sampaio, L. A., 2009: Benzocaína e eugenol como anestésicos para juvenis do pampo Trachinotus marginatus. Ciênc. Rural 39, 866– 870. Otto, D. M. E.; Sen, C. K.; Casley, W. L.; Moon, T. W., 1997: Regulation of 3,30,4,40-tetrachlorobiphenyl induced cytochrome P450 metabolism by thiols in tissues of rainbow trout. Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 117(C), 299– 309. Packer, L.; Tritschler, H. J.; Wessel, K., 1997: Neuroprotection by the metabolic antioxidant α-lipoic acid. Free Radic. Biol. Med 22, 359– 378. Packer, L.; Kraemer, K.; Rimbach, G., 2001: Molecular aspects of lipoic acid in the prevention of diabetes complications. Nutrition. 17, 888– 895. Park, K. H.; Terjesen, B. F.; Tesser, M. B.; Portella, M. C.; Dabrowski, K., 2006: α-Lipoic acid enrichment partially reverses tissue ascorbic acid depletion in pacu (Piaractus mesopotamicus) fed vitamin C-devoid diets. Fish Physiol. Biochem. 32, 329– 338. Pharm, R. T.; Gardner, J. L.; Gallagher, E. P., 2002: Conjugation of 4-hydroxynonenal by largemouth bass (Micropterus salmoides) glutathione S-transferases. Mar. Environ. Res. 54, 291– 295. Reed, L. J.; Florkin, M.; Stotz, E. H., 1974: Multienzyme complexes. Acc. Chem. Res. 7, 40– 46. Sampaio, L. A.; Tesser, M. B.; Burkert, D., 2003: Tolerância de juvenis do pampo Trachinotus mar-ginatus (Teleostei, Carangidae) ao choque agudo de salinidade em laboratório. Ciênc. Rural 33, 757– 761. Sies, H., 1991: Oxidative stress: oxidants and antioxidants. Ed.: H. Sies, Academic Press, London, 650pp. ISBN 0126427623. Simbula, G.; Columbano, A.; Ledda-Columbano, G. M.; Sanna, L.; Deidda, M.; Adiana, A.; Pibiri, M., 2007: Increased ROS generation and p53 activation in a-lipoic acid induced apoptosis of hepatoma cells. Apoptosis 12, 113– 123. Suh, J. H.; Shenvi, S. V.; Dixon, B. M.; Liu, H.; Jaiswal, A. K.; Liu, R.-M.; Hagen, T. M., 2004: Decline in transcriptional activity of Nrf2 causes age-related loss of glutathione synthesis, which is reversible with lipoic acid. Proc. Natl. Acad. Sci. U. S. A. 101, 3381– 3386. Terjesen, B. F.; Park, K.; Tesser, M. B.; Portella, M. C.; Zhang, Y.; Dabrowski, K., 2004: Lipoic acid and ascorbic acid affect plasma free amino acids selectively in the teleost fish Pacu (Piaractus mesopotamicus). J. Nutr. 134, 2930– 2934. Trattner, S.; Pickova, J.; Park, K. H.; Rinchard, J.; Dabrowski, K., 2007: Effects of α-lipoic and ascorbic acid on the muscle and brain fatty acids and antioxidant profile of the South American pacu Piaractus mesopotamicus. Aquaculture 273, 158– 164. UNESCO, 1983. Chemical methods for use in marine environmental monitoring. Manual and Guides 12, Intergovernmental Oceanographic Commission. Paris, France. Valdecantos, M. P.; Perez-Matute, P.; Quintero, P.; Martinez, J. A., 2010: Vitamin C, resveratrol and lipoic acid actions on isolated rat liver mitochondria: all antioxidants but different. Redox Rep. 15, 207– 216. Wang, Z. J.; Luo, D. H., 2007: Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino. Carbohyd. Polym. 68, 54– 58. Zar, J. H., 1984: Biostatistical analysis, 2nd edn. Prentice Hall, New Jersey. Englewood Cliffs, New Jersey. pp. 162– 184. Zhang, W.; Chen, Q.; Mai, K.; Xu, W.; Wang, X.; Liufu, Z., 2010: Effects of dietary α-lipoic acid on the growth and antioxidative responses of juvenile abalone Haliotis discus hannai Ino. Aquacult. Res. 41, 781– 787. Zhou, J.; Wang, W.; Wang, A.; He, W.; Zhou, Q.; Liu, Y.; Xu, J., 2009: Glutathione S-transferase in the white shrimp Litopenaeus vannamei: characterization and regulation under pH stress. Comp. Biochem. Physiol. 150(C), 224– 230. Citing Literature Volume29, Issue5October 2013Pages 1123-1128 ReferencesRelatedInformation
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