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DNA–Polymer Micelles as Nanoparticles with Recognition Ability

2011; Wiley; Volume: 17; Issue: 48 Linguagem: Inglês

10.1002/chem.201101561

1521-3765

Renée Mayap Talom, Gad Fuks, Leonard Kaps, Julian Oberdisse, Christel Cerclier, Cédric Gaillard, Christophe Mingotaud, Fabienne Gauffre,

DNA and Nucleic Acid Chemistry

Abstract The Watson–Crick binding of DNA single strands is a powerful tool for the assembly of nanostructures. Our objective is to develop polymer nanoparticles equipped with DNA strands for surface‐patterning applications, taking advantage of the DNA technology, in particular, recognition and reversibility. A hybrid DNA copolymer is synthesized through the conjugation of a ssDNA (22‐mer) with a poly(ethylene oxide)‐poly(caprolactone) diblock copolymer (PEO‐ b ‐PCl). It is shown that, in water, the PEO‐ b ‐PCl‐ssDNA 22 polymer forms micelles with a PCl hydrophobic core and a hydrophilic corona made of PEO and DNA. The micelles are thoroughly characterized using electron microscopy (TEM and cryoTEM) and small‐angle neutron scattering. The binding of these DNA micelles to a surface through DNA recognition is monitored using a quartz crystal microbalance and imaged by atomic force microscopy. The micelles can be released from the surface by a competitive displacement event.