The Drosophila ets-2 gene: Molecular structure, chromosomal localization, and developmental expression
1988; Elsevier BV; Volume: 127; Issue: 1 Linguagem: Inglês
10.1016/0012-1606(88)90187-x
ISSN1095-564X
AutoresLouis J. Pribyl, Dennis K. Watson, Mary Jane McWilliams, Richard Ascione, Takis S. Papas,
Tópico(s)interferon and immune responses
ResumoThe cellular homologs of the ets gene from the avian erythroblastosis retrovirus E26 have been studied in chickens, humans, mice, and cats. In this report a further evolutionary step is taken by isolating and characterizing a Drosophila ets-related genomic clone. Sequence analysis of this clone has shown it to contain the 3′ end of the v-ets gene, called ets-2, corresponding to the last two exons of chicken ets. The predicted amino acid sequence was found to have over 90% homology when compared to that of v-ets. This is the highest level of conservation observed for any previously characterized Drosophila oncogene homolog. Expression of the ets-2 gene occurs throughout development, but is highest during the embryonic and pupal stages. By in situ hybridization, the ets-2 chromosomal position was determined to be 58AB which corresponds to no known phenotypic mutant. As this is a highly conserved gene, the Drosophila model system should prove useful for the determination of the ets gene function.
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