Portal de Periódicos da CAPES

15-Deoxy-Δ12,14-prostaglandin J2 Inhibits IL-10 and IL-12 Production by Macrophages

2001; Elsevier BV; Volume: 283; Issue: 2 Linguagem: Inglês

10.1006/bbrc.2001.4783

1090-2104

Yasu‐Taka Azuma, Mitsuko Shinohara, Pao‐Li Wang, Kiyoshi Ohura,

NF-κB Signaling Pathways

15-Deoxy-Δ12,14-prostaglandin J2 (dPGJ2) is a metabolite of prostaglandin D2, that binds to peroxisome proliferator-activated receptor γ (PPARγ). PPARγ and prostaglandin D2 synthase, which is required for dPGJ2 synthesis, are predominantly expressed in macrophages. In contrast, IL-10 and IL-12 produced by macrophages stimulate Th1 and Th2 immune response, respectively. This study investigated the effect of dPGJ2 on IL-10 and IL-12 production by macrophages in response to lipopolysaccharide (LPS). Our data clearly demonstrated that dPGJ2 inhibits LPS-induced IL-10 and IL-12 production by macrophages. A different agonist of PPARγ, 13-hydroxyoctadecadienoic acid, similarly inhibited the production of IL-10 and IL-12 in response to LPS. Further, dPGJ2 did not appear to act through the PGD2 receptor. These results suggest that dPGJ2 may inhibit LPS-induced IL-10 and IL-12 production by macrophages through PPARγ.