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Efeitos da lipoproteína LDL-oxidada sobre a proliferação e a motilidade espontânea in vitro de células endoteliais de artérias coronárias humanas

2004; Sociedade Brasileira de Cardiologia (SBC); Volume: 83; Issue: 6 Linguagem: Inglês

10.1590/s0066-782x2004001800007

1678-4170

Hermes Tóros Xavier, Dulcinéia Saes Parra Abdalla, Tânia Leme da Rocha Martinez, José Antônio Franchini Ramires, Antônio Ricardo de Toledo Gagliardi,

Atherosclerosis and Cardiovascular Diseases

To investigate the effects of low concentrations of oxidized LDL (oxLDL) on the proliferation and spontaneous motility of human coronary artery endothelial cells (HCAEC) in culture.Cultures of HCAEC were treated with low concentrations of native LDL (nLDL) isolated from human plasma and with LDL minimally oxidized through different chemical methods; the effects were compared.Native LDL had no deleterious effects on in vitro proliferation and motility of HCAEC; however, at its highest concentration and for a longer exposure, nLDL inhibited cell proliferation. The LDL chemically oxidized by spermine nonoate (SNO) and 3-morpholinylsydnonimine (SYN-1) had significant inhibiting effects on in vitro proliferation and motility of HCAEC, which were proportional to the greatest concentrations and degrees of oxidation of LDL.OxLDL has a cytotoxic effect, inhibiting the proliferation and spontaneous motility of HCAEC in culture. This effect is proportional to the concentration and degree of oxidation of LDL; native LDL is relatively innocuous.