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Stereoselective determination of demethyl‐ and didemethyl‐citalopram in rat plasma and brain tissue by liquid chromatography with fluorescence detection using precolumn derivatization

2007; Wiley; Volume: 22; Issue: 3 Linguagem: Inglês

10.1002/bmc.922

1099-0801

Silvia Millán‐Martín, M. Aránzazu Goicolea, A. Sánchez, Alberto Gómez‐Caballero, M. Carmen Sampedro, Nora Unceta, Ramón J. Barrio,

Analytical Methods in Pharmaceuticals

Abstract A rapid and sensitive HPLC enantioselective method with fluorescence detection was developed to determine (−)‐( R ) and (+)‐( S ) enantiomers of the metabolites of citalopram, demethyl‐ and didemethyl‐citalopram in plasma and brain tissue. This assay involves pre‐column chiral derivatization with (−)‐( R )‐1‐(1‐naphthyl)ethyl isocyanate followed by separation on a normal‐phase silica column. The developed liquid–liquid extraction procedure permits quantitative determination of analytes with recoveries ranged between 81 and 88% with intra‐ and inter‐day relative standard deviations less than 10.5%. Linearity was obtained over the concentration range 5–1000 ng/mL and 100–10,000 ng/g for spiked drug‐free plasma and brain tissue, respectively, with detection limits lower than 2.1 ng/mL and 42.8 ng/g. Copyright © 2007 John Wiley & Sons, Ltd.