Carboxymethylation of a polysaccharide extracted from Ganoderma lucidum enhances its antioxidant activities in vitro
2009; Elsevier BV; Volume: 78; Issue: 2 Linguagem: Inglês
10.1016/j.carbpol.2009.03.028
ISSN1879-1344
AutoresJing Xu, Wei Liu, Wenbing Yao, Xiubing Pang, Dengke Yin, Xiangdong Gao,
Tópico(s)Seaweed-derived Bioactive Compounds
ResumoThe chemical carboxylmethylated polysaccharide (C-GLP), which derived from water-insoluble crude Ganoderma lucidum polysaccharide (GLP), was prepared. Water solubility, chemical characterization, and antioxidant activities in vitro of C-GLP were determined. The solubility of C-GLP in distilled water reached 100 mg/ml, which was much higher than the solubility of GLP. Chemical analysis indicated that C-GLP was composed of Glc:Man:Gal = 33.0:1.0:3.4 with a molecular weight of 1.8 × 106 Da and a carboxymethyl content of 11.07%. The signals of carboxymethyl were found in IR and 13C NMR spectra. Moreover, a high antioxidant activity of C-GLP was observed, especially in scavenging of hydroxyl radical (83.7% at 5 mg/ml) and hydrogen peroxide (51.6% at 10 mg/ml). This study indicates the effects of carboxymethylation on water-insoluble polysaccharide and explores a potential antioxidant in food industry and pharmaceuticals.
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