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Electroporation of Cells in Microfluidic Droplets

2009; American Chemical Society; Volume: 81; Issue: 5 Linguagem: Inglês

10.1021/ac9001172

1520-6882

Yihong Zhan, Jun Wang, Ning Bao, Chang Lu,

Microfluidic and Bio-sensing Technologies

Droplet-based microfluidics has raised a lot of interest recently due to its wide applications to screening biological/chemical assays with high throughput. Despite the advances on droplet-based assays involving cells, gene delivery methods that are compatible with the droplet platform have been lacking. In this report, we demonstrate a simple microfluidic device that encapsulates cells into aqueous droplets and then electroporates the encapsulated cells. The electroporation occurs when the cell-containing droplets (in oil) flow through a pair of microelectrodes with a constant voltage established in between. We investigate the parameters and characteristics of the electroporation. We demonstrate delivering enhanced green fluorescent protein (EGFP) plasmid into Chinese hamster ovary (CHO) cells. We envision the application of this technique to high-throughput functional genomics studies based on droplet microfluidics.