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Fast nonlinear spectral microscopy of in vivo human skin

2011; Optica Publishing Group; Volume: 2; Issue: 2 Linguagem: Inglês

10.1364/boe.2.000365

2156-7085

Arjen N. Bader, Ana‐Maria Pena, C. Johan van Voskuilen, Jonathan A. Palero, Frédéric Leroy, Anne Colonna, Hans C. Gerritsen,

Spectroscopy Techniques in Biomedical and Chemical Research

An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.