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Influence of serum factors on the interaction of concanavalin a with inert and biological surfaces

1977; Elsevier BV; Volume: 106; Issue: 1 Linguagem: Inglês

10.1016/0014-4827(77)90237-3

1090-2422

Philip G. Phillips, Martin Lubin,

Platelet Disorders and Treatments

The plastic surfaces of tissue culture dishes bind about 5 × l02 molecules of concanavalin A (ConA)/μ2. This binding is virtually uninhibited by alpha-methyl-mannoside (α-MM). If the plastic is treated with fetal calf or dog serum, binding of ConA increases to about 8 × 103 molecules/μ2, but is almost completely inhibited by α-MM. The level of binding of ConA to human red blood cells (RBC)—about 1.4 × 104 molecules/μ2—does not increase if cells are first treated with fetal calf or dog serum; human RBC treated with dog serum, however, are about 100 times more agglutinable by ConA than are untreated cells. Our results show that: (a) serum components can increase the binding of ConA to inert surfaces; (b) serum components can increase the sensitivity of human RBC to agglutination by ConA; and (c) ConA can bind to surfaces by at least two mechanisms (one involving sites sensitive to inhibition by α-MM, and the other not).