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Preparative separation of pyrogens from proteins by isoelectric focusing using a multicompartment electrolyser with Immobiline membranes

1990; Wiley; Volume: 11; Issue: 11 Linguagem: Inglês

10.1002/elps.1150111120

1522-2683

James E. Lucas, Michel Faupel, C. Goecking,

thermodynamics and calorimetric analyses

Abstract Due to the nature of lipopolysaccharide endotoxin structures of bacterial pyrogens, their removal from solutions containing therapeutic proteins is often a problem in the pharmaceutical industry. In this report we describe the application of electromotive force to dislodge lipopolysaccharide endotoxins from proteins. This was performed by employing a multicompartment electrolyzer fitted with Immobiline membranes of specified p I s. A thousand‐fold reduction of endotoxin could be achieved in the model test system described. This contribution describes the use of a new recycling isoelectric focusing approach without the use of carrier ampholytes.