Baculovirus virions displaying Plasmodium berghei circumsporozoite protein protect mice against malaria sporozoite infection
2003; Elsevier BV; Volume: 316; Issue: 1 Linguagem: Inglês
10.1016/j.virol.2003.08.003
ISSN1096-0341
AutoresShigeto Yoshida, Daisuke Kondoh, Eriko Arai, Hiroyuki Matsuoka, Chisato Seki, Takao Tanaka, Masaji Okada, Akira Ishii,
Tópico(s)Protein purification and stability
ResumoThe display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein–protein interactions and for cell-specific targeting in gene transfer applications. To evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (AcNPV-CSPsurf) that displays rodent malaria Plasmodium berghei circumsporozoite protein (PbCSP) on the virion surface as a fusion protein with the major baculovirus envelope glycoprotein gp64. The PbCSP-gp64 fusion protein was incorporated and oligomerized on the virion surface and led to a 12-fold increase in the binding activity of AcNPV-CSPsurf virions to HepG2 cells. Immunization with adjuvant-free AcNPV-CSPsurf virions induced high levels of antibodies and gamma interferon-secreting cells against PbCSP and protected 60% of mice against sporozoite challenge. These data demonstrate that AcNPV-CSPsurf displays sporozoite-like PbCSP on the virion surface and possesses dual potentials as a malaria vaccine candidate and a liver-directed gene delivery vehicle.
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