Induction of Blood‐Brain Barrier Endothelial Cell Differentiation a
1991; Wiley; Volume: 633; Issue: 1 Linguagem: Inglês
10.1111/j.1749-6632.1991.tb15630.x
ISSN1749-6632
Autores Tópico(s)Apelin-related biomedical research
ResumoAnnals of the New York Academy of SciencesVolume 633, Issue 1 p. 405-419 Induction of Blood-Brain Barrier Endothelial Cell Differentiationa WERNER RISAU, WERNER RISAU Max-Planck-Institut für Psychiatrie Am Klopferspitz 18a D-8033 Martinsried, FRGSearch for more papers by this author WERNER RISAU, WERNER RISAU Max-Planck-Institut für Psychiatrie Am Klopferspitz 18a D-8033 Martinsried, FRGSearch for more papers by this author First published: December 1991 https://doi.org/10.1111/j.1749-6632.1991.tb15630.xCitations: 74 a This research is supported by the Max-Planck Gesellschaft and the Deutsche Forschungsgemeinschaft. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES 1 Majno, G. 1965. Ultrastructure of the vascular membrane. In Handbook of Physiology, Circulation III. W. F. Hamilton & P. Dow, eds.: 2293–2375. Washington . American Physiology Society. Google Scholar 2 Reese, T. S. & M. J. Karnovsky. 1967. Fine structural localization of a blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34: 207–217. 10.1083/jcb.34.1.207 CASPubMedWeb of Science®Google Scholar 3 Brightman, M. W. & T. S. Reese. 1969. Junctions between intimately apposed cell membranes in the vertebrate brain. J. Cell Biol. 40: 648–677. 10.1083/jcb.40.3.648 CASPubMedWeb of Science®Google Scholar 4 Goldstein, G. W. & A. L. Betz. 1986. The blood-brain barrier. Sci. Am. 255: 70–79. 10.1038/scientificamerican0986-74 Web of Science®Google Scholar 5 Risau, W. & H. Wolburg. 1990. Development of the blood-brain barrier. Trends Neurosci. 13: 174–178. 10.1016/0166-2236(90)90043-A CASPubMedWeb of Science®Google Scholar 6 Betz, A. L. & G. W. Goldstein. 1986. Specialized properties and solute transport in brain capillaries. Ann. Rev. Physiol. 48: 241–250. 10.1146/annurev.ph.48.030186.001325 CASPubMedWeb of Science®Google Scholar 7 Hardebo, J. E. & C. Owman. 1980. Barrier mechanisms for neurotransmitter monoamines and their precursors at the blood-brain barrier interface. Ann. Neurol. 8: 1–11. 10.1002/ana.410080102 CASPubMedWeb of Science®Google Scholar 8 Leonhardt, H., Ependym und circumventriculäre Organe. 1980. In Handbuch der mikroskopischen Anatomie des Menschen. A. Oksche & L. Vollrath, eds.: 177–666. Springer. Berlin , Heidelberg , New York . Google Scholar 9 Bouchaud, C. & O. Bosler. 1986. The circumventricular organs of the mammalian brain with special reference to monoaminergic innervation. Int. Rev. Cytol. 105: 283–327. 10.1016/S0074-7696(08)61066-9 CASPubMedWeb of Science®Google Scholar 10 Bär, T. 1980. The vascular system of the cerebral cortex. Adv. Anat. Embryol. Cell Biol. 59: 1–62. 10.1007/978-3-642-67432-7_1 Google Scholar 11 Feeney, J. F. Jr. & R. L. Watterson. 1946. The development of the vascular pattern within the walls of the central nervous system of the chick embryo. J. Morphol. 78: 231–303. 10.1002/jmor.1050780205 PubMedWeb of Science®Google Scholar 12 Robertson, P. L., M. Du Bois, P. D. Bowman & G. W. Goldstein. 1985. Angiogenesis in developing rat brain: An in vivo and in vitro study. Dev. Brain Res. 23: 219–223. 10.1016/0165-3806(85)90044-6 CASWeb of Science®Google Scholar 13 Risau, W. 1986. Developing brain produces an angiogenesis factor. Proc. Natl. Acad. Sci. USA 83: 3855–3859. 10.1073/pnas.83.11.3855 CASPubMedWeb of Science®Google Scholar 14 Risau, W., P. Gautschi-Sova & P. Böhlen. 1988. Endothelial cell growth factors in embryonic and adult chick brain are related to human acidic fibroblast growth factors. EMBO J. 7: 959–962. 10.1002/j.1460-2075.1988.tb02901.x CASPubMedWeb of Science®Google Scholar 15 Stewart, P. A. & M. J. Wiley. 1981. Developing nervous tissue induces formation of blood-brain barrier characteristics in invading endothelial cells: A study using quailchick transplantation chimeras. Dev. Biol. 84: 183–192. 10.1016/0012-1606(81)90382-1 PubMedWeb of Science®Google Scholar 16 Risau, W., R. Hallmann, U. Albrecht & S. Henke-Fahle. 1986. Brain induces the expression of an early cell surface marker for blood-brain barrier specific endothelium. EMBO J. 5: 3179–3183. 10.1002/j.1460-2075.1986.tb04627.x CASPubMedWeb of Science®Google Scholar 17 Wakai, S. & N. Hirokawa. 1978. Development of the blood-brain barrier to horseradish peroxidase in the chick embryo. Cell Tissue Res. 195: 195–203. 10.1007/BF00236719 CASPubMedWeb of Science®Google Scholar 18 Delorme, P., J. Gayet & G. Grignon. 1970. Ultrastructural study on transcapillary exchanges in the developing telencephalon of the chicken. Brain Res. 22: 269–283. 10.1016/0006-8993(70)90471-3 CASPubMedWeb of Science®Google Scholar 19 Risau, W., R. Hallmann & U. Albrecht. 1986. Differentiation-dependent expression of protein in brain endothelium during development of the blood-brain barrier. Dev. Biol. 117: 537–545. 10.1016/0012-1606(86)90321-0 CASPubMedWeb of Science®Google Scholar 20 MØllgård, K. & N. R. Saunders. 1986. The development of the human blood-brain and blood-CSF barriers. Neuropath. Appl. Neurobiol. 12: 337–358. 10.1111/j.1365-2990.1986.tb00146.x CASPubMedWeb of Science®Google Scholar 21 Saunders, N. R., K. M. Dziegielewska & K. Mollgard. 1991. Letter to the editor. Trends Neurosci. 14: 14. 10.1016/0166-2236(91)90176-U CASPubMedWeb of Science®Google Scholar 22 Risau, W. & H. Wolburg. 1991. Reply. Trends Neurosci. 14: 15. 10.1016/0166-2236(91)90177-V Web of Science®Google Scholar 23 Crone, C. & S.-P. Olesen. 1982. Electrical resistance of brain microvascular endothelium. Brain Res. 241: 49–55. 10.1016/0006-8993(82)91227-6 CASPubMedWeb of Science®Google Scholar 24 Nagy, Z., H. Peters & I. Hüttner. 1984. Fracture faces of cell junctions in cerebral endothelium during normal and hyperosmotic conditions. Lab. Invest. 50: 313–322. CASPubMedWeb of Science®Google Scholar 25 Cordon-Cardo, C., J. P. O'Brien, D. Casals, L. Rittman-Grauer, J. L. Biedler, M. R. Melamed & J. R. Bertino. 1989. Multidrug-resistance gene (P-glycoprotein) is expressed by endothelial cells at blood-brain barrier sites. Proc. Natl. Acad. Sci. USA 86: 695–698. 10.1073/pnas.86.2.695 CASPubMedWeb of Science®Google Scholar 26 Broadwell, R. D. 1989. Transcytosis of macromolecules through the blood-brain barrier: A cell biological perspective and critical appraisal. Acta Neuropathol. 79: 117–128. 10.1007/BF00294368 PubMedWeb of Science®Google Scholar 27 Duffy, K. R. & W. M. Pardridge. 1987. Blood-brain barrier transcytosis of insulin in developing rabbits. Brain Res. 420: 32–38. 10.1016/0006-8993(87)90236-8 CASPubMedWeb of Science®Google Scholar 28 Meresse, S., C. Delbart, J. C. Fruchart & R. Cecchelli. 1989. Low-density lipoprotein receptor on endothelium of brain capillaries. J. Neurochem. 53: 340–345. 10.1111/j.1471-4159.1989.tb07340.x CASPubMedWeb of Science®Google Scholar 29 Mostov, K. E. & N. E. Simister. 1985. Transcytosis. Cell 43: 389–390. 10.1016/0092-8674(85)90166-7 CASPubMedWeb of Science®Google Scholar 30 Jefferies, W. A., M. R. Brandon, S. V. Hunt, A. F. Williams, K. C. Gatter & D. Y. Mason. 1984. Transferrin receptor on endothelium of brain capillaries. Nature 312: 162–163. 10.1038/312162a0 CASPubMedWeb of Science®Google Scholar 31 Orlowski, M., G. Sessa & J. P. Green. 1974. Gamma-glutamyl transpeptidase in brain capillaries: Possible site of a blood-brain barrier for amino acids. Science 184: 66–68. 10.1126/science.184.4132.66 CASPubMedWeb of Science®Google Scholar 32 Leduc, E. H. & G. B. Wislocki. 1952. The histochemical localization of acid and alkaline phosphatases, non-specific esterase and succinic dehydrogenase in the structures comprising the hematoencephalic barrier of the rat. J. Comp. Neurol. 97: 242–279. 10.1002/cne.900970203 Web of Science®Google Scholar 33 Joö, F. & B. Csillik. 1966. Topographic correlation between the hemato-encephalic barrier and the cholinesterase activity of brain capillaries. Exp. Brain Res. 1: 147–151. 10.1007/BF00236867 CASPubMedWeb of Science®Google Scholar 34 Michalak, T., F. P. White, A. L. Gard & G. R. Dutton. 1986. A monoclonal antibody to the endothelium of rat brain microvessels. Brain Res. 379: 320–328. 10.1016/0006-8993(86)90786-9 CASPubMedWeb of Science®Google Scholar 35 Sternberger, N. H. & L. A. Sternberger. 1987. Blood-brain barrier protein recognized by monoclonal antibody. Proc. Natl. Acad. Sci. USA 84: 8169–8173. 10.1073/pnas.84.22.8169 CASPubMedWeb of Science®Google Scholar 36 Pardridge, W. M., J. Yang, J. Eisenberg & L. J. Mietus. 1986. Antibodies to blood-brain barrier bind selectively to brain capillary endothelial lateral membranes and to a 46K protein. J. Cereb. Blood Flow Metab. 6: 203–211. 10.1038/jcbfm.1986.33 CASPubMedWeb of Science®Google Scholar 37 Gaffney, J., D. West, F. Arnold, A. Sattar & S. Kumar. 1985. Differences in the uptake of modified low density lipopoteins by tissue cultured endothelial cells. J. Cell Sci. 79: 317–325. CASPubMedWeb of Science®Google Scholar 38 Ishii, H., H. H. Salem, C. E. Bell, E. A. Laposata & P. W. Majerus. 1986. Thrombomodulin, an endothelial anticoagulant protein, is absent from the human brain. Blood 67: 362–365. 10.1182/blood.V67.2.362.362 CASPubMedWeb of Science®Google Scholar 39 Robinson, A. P., T. M. White & D. W. Mason. 1986. MRC OX-43: A monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries. Immunology 57: 231–237. CASPubMedWeb of Science®Google Scholar 40 Vorbrodt, A. W., A. S. Lossinsky & H. M. Wisniewski. 1986. Localization of alkaline phosphatase activity in endothelial of developing and mature mouse blood-brain barrier. Dev. Neurosci. 8: 1–13. 10.1159/000112236 CASPubMedWeb of Science®Google Scholar 41 Stewart, P. A. & E. M. Hayakawa. 1987. Interendothelial junctional changes underlie the developmental 'tightening' of the blood-brain barrier. Dev. Brain Res. 32: 271–281. 10.1016/0165-3806(87)90107-6 Web of Science®Google Scholar 42 Sims, D. E. 1986. The Pericyte–A review. Tissue & Cell 18: 153–174. 10.1016/0040-8166(86)90026-1 PubMedWeb of Science®Google Scholar 43 Farrell, C. R., P. A. Stewart, C. L. Farrell & R. F. Del Maestro. 1987. Pericytes in human cerebral microvasculature. Anat. Rec. 218: 466–469. 10.1002/ar.1092180416 CASPubMedWeb of Science®Google Scholar 44 Simionescu, M. Ghinea, A. Fixman, M. Lasser, L. Kukes, N. Simionescu & G. E. Palade. 1988. The cerebral microvasculature of the rat: Structure and luminal surface properties during early development. J. Submicrosc. Cytol. 20: 243–261. PubMedWeb of Science®Google Scholar 45 Krause, D., B. Vatter & R. Dermietzel. 1988. Immunochemical and immunocytochemical characterization of a novel monoclonal antibody. Cell Tissue Res. 252: 543–555. 10.1007/BF00216641 CASPubMedWeb of Science®Google Scholar 46 Jotereau, F. & N. M. Le Douarin. 1978. The developmental relationship between osteocytes and osteoclasts: A study using the quail-chick nuclear marker in endochondral ossification. Dev. Biol. 63: 253–265. 10.1016/0012-1606(78)90132-X CASPubMedWeb of Science®Google Scholar 47 Pardanaud, L., F. Yassine & F. Dieterlenlievre. 1989. Relationship between vasculogenesis, angiogenesis and hematopoiesis during avian ontogeny. Development 105: 473–485. 10.1242/dev.105.3.473 CASPubMedWeb of Science®Google Scholar 48 Phelps, C. H. 1972. The development of glio-vascular relationships in the rat spinal cord. Z. Zellforsch. 128: 555–563. 10.1007/BF00306988 CASPubMedWeb of Science®Google Scholar 49 Senjo, M., T. Ishibashi, T. Terashima & Y. Inoue. 1986. Correlation between astrogliogenesis and blood-brain barrier formation: Immunocytochemical demonstration by using astroglia-specific enzyme glutathione S-transferase. Neurosci. Lett. 66: 39–42. 10.1016/0304-3940(86)90162-X CASPubMedWeb of Science®Google Scholar 50 Janzer, R. C. & M. C. Raff. 1987. Astrocytes induce blood-brain barrier properties in endothelial cells. Nature 325: 253–257. 10.1038/325253a0 CASPubMedWeb of Science®Google Scholar 51 Greig, N. H. 1988. In Implications of the Blood-Brain Barrier and Its Manipulation. E. A. Neuwelt, ed.: 77–106. Plenum Medical Book Company. New York . Google Scholar 52 Newman, E. A. 1985. J. Neurosci. 5: 2225–2239. 10.1523/JNEUROSCI.05-08-02225.1985 CASPubMedWeb of Science®Google Scholar 53 Nilius, B. & A. Reichenbach. 1988. Pfluegers Arch. 411: 654–660. 10.1007/BF00580862 CASPubMedWeb of Science®Google Scholar 54 Wolburg, H. & K. Berg. 1988. Glia 1: 246–252. 10.1002/glia.440010403 CASPubMedWeb of Science®Google Scholar 55 Joó, F. 1985. The blood-brain barrier in vitro: Ten years of research on microvessels isolated from the brain. Neurochem. Int. 7: 1–25. 10.1016/0197-0186(85)90002-6 CASPubMedWeb of Science®Google Scholar 56 Rutten, M. J., R. L. Hoover & M. J. Karnovsky. 1987. Electrical resistance and macromolecular permeability of brain endothelial monolayer cultures. Brain Res. 425: 301–310. 10.1016/0006-8993(87)90513-0 CASPubMedWeb of Science®Google Scholar 57 Carson, M. P. & C. C. Haudenschild. 1986. Microvascular endothelium and pericytes: High yield, low passage cultures. In Vitro Cell Dev. Biol. 22: 344–354. 10.1007/BF02623409 CASPubMedWeb of Science®Google Scholar 58 Dehouck, M. P., S. Meresse, P. Delorme, J. C. Fruchart & R. Cecchelli. 1990. An easier, reproducible, and mass-production method to study the blood-brain-barrier in vitro. J. Neurochem. 54: 1798–1801. 10.1111/j.1471-4159.1990.tb01236.x CASPubMedWeb of Science®Google Scholar 59 Williams, S. K., J. F. Gillis, M. A. Matthews, R. C. Wagner & M. W. Bitensky. 1980. Isolation and characterization of brain endothelial cells: Morphology and enzyme activity. J. Neurochem. 35: 374–381. 10.1111/j.1471-4159.1980.tb06274.x CASPubMedWeb of Science®Google Scholar 60 Mischeck, U., J. Meyer & H.-J. Galla. 1989. Characterization of gamma-glutamyl transpeptidase activity of cultured endothelial cells from porcine brain capillaries. Cell Tissue Res. 256: 221–226. 10.1007/BF00224737 CASPubMedWeb of Science®Google Scholar 61 Risau, W., B. Engelhardt & H. Wekerle. 1990. Immune function of the blood-brain barrier: Incomplete presentation of protein (auto-)antigens by rat brain microvascular endothelium in vitro. J. Cell Biol. 110: 1757–1766. 10.1083/jcb.110.5.1757 CASPubMedWeb of Science®Google Scholar 62 Rupnick, M. A., A. Carey & S. K. Williams. 1988. Phenotypic diversity in cultured cerebral microvascular endothelial cells. In Vitro Cell Dev. Biol. 24: 435–444. 10.1007/BF02628495 PubMedWeb of Science®Google Scholar 63 Meresse, S., M. P. Dehouck, P. Delorme, M. Bensaid, J. P. Tauber, C. Delbart, J. C. Fruchart & R. Cecchelli. 1989. Bovine brain endothelial-cells express tight junctions and monoamine-oxidase activity in long-term culture. J. Neurochem. 53: 1363–1371. 10.1111/j.1471-4159.1989.tb08526.x CASPubMedWeb of Science®Google Scholar 64 Risau, W., A. Dingler, U. Albrecht, M.-P. Dehouck & R. Cecchelli. 1991. Blood-brain barrier pericytes are the main source of gamma-glutamyltranspeptidase activity in brain capillaries. J. Neurochem. Submitted. Google Scholar 65 Tao-Cheng, J.-H. & M. W. Brightman. 1988. Development of membrane interactions between brain endothelial cells and astrocytes in vitro. Int. J. Dev. Neurosci. 6: 25–37. 10.1016/0736-5748(88)90026-3 CASPubMedWeb of Science®Google Scholar 66 DeBault, L. E. & P. A. Cancilla. 1980. Gamma-glutamyl transpeptidase in isolated brain endothelial cells: Induction by glial cells in vitro. Science 207: 653–655. 10.1126/science.6101511 CASPubMedWeb of Science®Google Scholar 67 Smith, Q. R., S. Momma, M. Aoyagi & S. I. Rapoport. 1987. Kinetics of neutral amino acid transport across the blood-brain barrier. J. Neurochem. 49: 1651–1658. 10.1111/j.1471-4159.1987.tb01039.x CASPubMedWeb of Science®Google Scholar 68 Kalaria, R. N. & S. I. Harik. 1988. Adenosine receptors and the nucleoside transporter in human brain vasculature. J. Cereb. Blood Flow Metab. 8: 32–39. 10.1038/jcbfm.1988.5 CASPubMedWeb of Science®Google Scholar 69 Meyer, J., U. Mischeck, M. Veyhl, K. Henzel & H. J. Galla. 1990. Blood-brain-barrier characteristic enzymatic-properties in cultured brain capillary endothelial-cells. Brain Res. 514: 305–309. 10.1016/0006-8993(90)91425-G CASPubMedWeb of Science®Google Scholar 70 Maxwell, K., J. A. Berliner & P. A. Cancilla. 1987. Induction of gamma-GTP in cultured cerebral endothelial cells by a product released by astrocytes. Brain Res. 410: 309–314. 10.1016/0006-8993(87)90329-5 CASPubMedWeb of Science®Google Scholar 71 Stewart, P. A. 1980. Histochemical absence of gamma-glutamyl transpeptidase in chick brain capillary endothelium. Exp. Neurol. 67: 442–446. 10.1016/0014-4886(80)90244-7 CASPubMedWeb of Science®Google Scholar 72 Albert, Z., M. Orlowski, Z. Rzucidlo & J. Orlowska. 1986. Studies on gammaglutamyl transpeptidase activity and its histochemical localization in the central nervous system of man and different animal species. Acta Histochem. 25: 312–320. Google Scholar 73 Stevenson, B. R., J. D. Siliciano, M. S. Mooseker & D. A. Goodenough. 1986. Identification of ZO-1: A high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J. Cell Biol. 103: 755–766. 10.1083/jcb.103.3.755 CASPubMedWeb of Science®Google Scholar 74 Stevenson, B. R., J. M. Anderson, D. A. Goodenough & M. S. Mooseker. 1988. Tight junction structure and ZO-1 content are identical in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance. J. Cell Biol. 107: 2401–2408. 10.1083/jcb.107.6.2401 CASPubMedWeb of Science®Google Scholar 75 Seulberger, H., F. Lottspeich & W. Risau. 1990. The inducible blood-brain barrier specific molecule HT7 is a novel immunoglobulin-like cell surface glycoprotein. EMBO J. 9: 2151–2158. 10.1002/j.1460-2075.1990.tb07384.x CASPubMedWeb of Science®Google Scholar 76 Albrecht, U., H. Seulberger, H. Schwarz & W. Risau. 1990. Correlation of blood-brain barrier function and HT7-protein distribution in chick brain circumventricular organs. Brain Res. 535: 49–61. 10.1016/0006-8993(90)91823-Y CASPubMedWeb of Science®Google Scholar 77 Fossum, S., S. Mallett & A. N. Barclay. 1991. The MRC OX-47 antigen of activated lymphocytes is a member of the immunoglobulin superfamily with an unusual transmembrane sequence. Eur. J. Immunol. 21: 671–679. 10.1002/eji.1830210320 CASPubMedWeb of Science®Google Scholar 78 Altruda, F., P. Cervella, M. L. Gaeta, A. Daniele, F. Giancotti, G. Tarone, G. Stefanuto & L. Silengo. 1989. Cloning of cDNA for a novel mouse membrane glycoprotein (gp42): Shared identity to histocompatibility antigens, immunoglobulins and neural-cell adhesion molecules. Gene 85: 445–452. 10.1016/0378-1119(89)90438-1 CASPubMedWeb of Science®Google Scholar 79 Schlosshauer, B. & K. H. Herzog. 1990. Neurothelin: An inducible cell-surface glycoprotein of blood-brain-barrier specific endothelial-cells and distinct neurons. J. Cell Biol. 110: 1261–1274. 10.1083/jcb.110.4.1261 CASPubMedWeb of Science®Google Scholar 80 Dick, A. P. K., S. I. Harik, A. Klip & D. M. Walker. 1984. Identification and characterization of the glucose transporter of the blood-brain barrier by cytochalasin B binding and immunological reactivity. Proc. Natl. Acad. Sci. USA 81: 7233–7237. 10.1073/pnas.81.22.7233 CASPubMedWeb of Science®Google Scholar 81 Flier, J. S., M. Mueckler, A. L. McCall & H. F. Lodsih. 1986. Distribution of glucose transporter messenger RNA transcripts in tissues of rat and man. J. Clin. Invest. 79: 657–661. 10.1172/JCI112864 PubMedWeb of Science®Google Scholar 82 Kalaria, R. N., S. A. Gravina, J. W. Schmidley, G. Perry & S. I. Harik. 1988. The glucose transporter of the human brain and blood-brain barrier. Ann. Neurol. 24: 757–764. 10.1002/ana.410240610 CASPubMedWeb of Science®Google Scholar 83 Gould, G. W. & G. I. Bell. 1990. Facilitative glucose transporters: An expanding family. Trends Biol. Sci. 15: 18–23. 10.1016/0968-0004(90)90125-U CASPubMedWeb of Science®Google Scholar 84 Harik, S. I., R. N. Kalaria, P. M. Whitney, L. Andersson, P. Lundahl, S. R. Ledbetter & G. Perry. 1990. Glucose transporters are abundant in cells with occluding junctions at the blood eye barriers. Proc. Natl. Acad. Sci. 87: 4261–4264. 10.1073/pnas.87.11.4261 CASPubMedWeb of Science®Google Scholar 85 Dingler, A. 1989. Untersuchungen von Blut-Hirn Schranken Proteinen an kultivierten Gehirnendothelzellen. University of Tubingen. Diploma Thesis. Google Scholar Citing Literature Volume633, Issue1Glial‐Neuronal InteractionDecember 1991Pages 405-419 ReferencesRelatedInformation
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