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Development of industrial production of poly-l-lactate from glucose

2001; Elsevier BV; Volume: 91; Issue: 6 Linguagem: Inglês

10.1016/s1389-1723(01)80185-7

1389-1723

Hitomi Ohara, Umeyuki Doi, Masataka Otsuka, Okuyama Hisashi, Sanae Okada,

Biochemical Acid Research Studies

Medium branched-chain esters can be used not only as a biofuel but are also useful chemicals with various industrial applications. The development of economically feasible and environment friendly bio-based fuels requires efficient cell factories capable of producing desired products in high yield. Herein, we sought to use a number of strategies to engineer Saccharomyces cerevisiae for high-level production of branched-chain esters. Mitochondrion-based expression of ATF1 gene in a base strain with an overexpressed valine biosynthetic pathway together with expression of mitochondrion-relocalized α-ketoacid decarboxylase (encoded by ARO10) and alcohol dehydrogenase (encoded by ADH7) not only produced isobutyl acetate, but also 3-methyl-1-butyl acetate and 2-methyl-1-butyl acetate. Further segmentation of the downstream esterification step into the cytosol to utilize the cytosolic acetyl-CoA pool for acetyltransferase (ATF)-mediated condensation enabled an additional fold improvement of ester productions. The best titre attained in the present study is 260.2 mg/L isobutyl acetate, 296.1 mg/L 3-methyl-1-butyl acetate and 289.6 mg/L 2-methyl-1-butyl acetate.