Pathogenic autoantibody production requires loss of tolerance against desmoglein 3 in both T and B cells in experimental pemphigus vulgaris
2002; Wiley; Volume: 32; Issue: 3 Linguagem: Inglês
10.1002/1521-4141(200203)32
ISSN1521-4141
AutoresKazuyuki Tsunoda, Takayuki Ota, Harumi Suzuki, Manabu Ohyama, Tetsuo Nagai, Takeji Nishikawa, Masayuki Amagai, Shigeo Koyasu,
Tópico(s)Coagulation, Bradykinin, Polyphosphates, and Angioedema
ResumoMechanisms of tolerance break against desmoglein 3 (Dsg3) in patients with pemphigus vulgaris (PV) producing pathogenic anti-Dsg3 IgG autoantibodies are unclear. In this study, using a novel PV mouse model involving Dsg3 knockout mice, we investigated the mechanisms leading to production of autoantibodies against Dsg3. Adoptive transfer of Dsg3–/– splenocytes immunized with recombinant mouse Dsg3 to Rag2–/– recipient mice expressing Dsg3 resulted in the stable production of anti-Dsg3 IgG and development of PV phenotypes including oral erosions with suprabasilar acantholysis. When purified T and B cells from Dsg3–/–, Dsg3+/– or Dsg3+/+ mice were mixed with various combinations and transferred to Rag2–/– mice, pathogenic anti-Dsg3 IgG production was observed only with a combination of Dsg3–/– T and Dsg3–/– B cells but not with the other combinations. These results suggest that loss of tolerance against Dsg3 in both B and T cells is important for the development of autoimmune state of PV.
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