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A new highly sensitive immunoassay for cytokines by dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA)

1992; Elsevier BV; Volume: 148; Issue: 1-2 Linguagem: Inglês

10.1016/0022-1759(92)90153-k

1872-7905

Atsushi Ogata, Hiromi Tagoh, Tae‐Ho Lee, Taro Kuritani, Yoshiyuki Takahara, T Shimamura, Hakuroh Ikegami, Masashi Kurimoto, Kazuyuki Yoshizaki, Tadamitsu Kishimoto,

Eosinophilic Disorders and Syndromes

Non-isotopic immunoassays for human tumor necrosis factor α (TNFα) and human interleukin-6 (IL-6) were established by employing the dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) system based on the time-resolved fluoroimmunoassay technique with europium-labeled antibody. Compared to enzyme-linked immunosorbent assays and bioassays, the sensitivity and range of measurement were significantly increased by applying the DELFIA systems to TNFα and IL-6. TNFα was measurable from 100 fg/ml to 10 ng/ml with the TNFα-DELFIA and IL-6 was measurable from 100 fg/ml to 1 ng/ml with the IL-6-DELFIA.