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Biochemical Characterization of Rab3-GTPase-activating Protein Reveals a Mechanism Similar to That of Ras-GAP

2000; Elsevier BV; Volume: 275; Issue: 41 Linguagem: Inglês

10.1074/jbc.m003705200

1083-351X

Aude Clabecq, Jean‐Pierre Henry, François Darchen,

Microtubule and mitosis dynamics

Small G proteins of the Rab family are regulators of intracellular vesicle traffic. Their intrinsic rate of GTP hydrolysis is very low but is enhanced by specific GTPase-activating proteins (GAPs) that switch G proteins to their inactive form. We have characterized the activity of recombinant Rab3-GAP on Rab3A in solution. The K m and K d values (75 μm) indicate a low affinity of Rab3-GAP for its substrate. The affinity is higher for the transition state analog Rab3A:GDP:AlFx (15 μm). Thek cat (1 s−1) is within the range of values reported for other GAPs. A mutation in the switch I region of Rab3A disrupted the interaction with Rab3-GAP. Furthermore, Rabphilin, a putative target of Rab3, inhibited the activity of Rab3-GAP on Rab3. Therefore, the Rab3-GAP-binding site involves the switch I region of Rab3 and overlaps with the Rabphilin-binding domain. Substitution of a single arginine residue (Arg-728) of Rab3-GAP disrupted its catalytic activity but not its interaction with Rab3A. We propose that Rab3-GAP, like Ras- and Rho-GAPs, stabilizes the transition state of Rab3 and provides a critical arginine residue to accelerate the GTPase reaction. Small G proteins of the Rab family are regulators of intracellular vesicle traffic. Their intrinsic rate of GTP hydrolysis is very low but is enhanced by specific GTPase-activating proteins (GAPs) that switch G proteins to their inactive form. We have characterized the activity of recombinant Rab3-GAP on Rab3A in solution. The K m and K d values (75 μm) indicate a low affinity of Rab3-GAP for its substrate. The affinity is higher for the transition state analog Rab3A:GDP:AlFx (15 μm). Thek cat (1 s−1) is within the range of values reported for other GAPs. A mutation in the switch I region of Rab3A disrupted the interaction with Rab3-GAP. Furthermore, Rabphilin, a putative target of Rab3, inhibited the activity of Rab3-GAP on Rab3. Therefore, the Rab3-GAP-binding site involves the switch I region of Rab3 and overlaps with the Rabphilin-binding domain. Substitution of a single arginine residue (Arg-728) of Rab3-GAP disrupted its catalytic activity but not its interaction with Rab3A. We propose that Rab3-GAP, like Ras- and Rho-GAPs, stabilizes the transition state of Rab3 and provides a critical arginine residue to accelerate the GTPase reaction. GTPase-activating protein guanosine 5′-(3-O-thio)triphosphate polymerase chain reaction glutathione S-transferase calmodulin Small GTP-binding proteins of the Rab family play a pivotal role in controlling membrane trafficking along the endo- and exocytic pathways (1Schimmoller F. Simon I. Pfeffer S.R. J. Biol. Chem. 1998; 273: 22161-22164Abstract Full Text Full Text PDF PubMed Scopus (270) Google Scholar, 2Chavrier P. Goud B. Curr. Opin. Cell Biol. 1999; 11: 466-475Crossref PubMed Scopus (422) Google Scholar). In their GDP-bound state, Rab proteins are bound to Rab-GDP dissociation inhibitor, which make them soluble in the cytosol. The activation process involves a poorly understood translocation step toward a specific membrane compartment correlated with the exchange of GTP for GDP. This reaction is catalyzed by specific guanine nucleotide exchange factors. GTP-bound Rab proteins then interact with various effectors, the list of which is rapidly growing. The functional cycle ends by GTP hydrolysis mediated by specific GTPase-activating proteins (GAPs)1 followed by membrane extraction by Rab-GDP dissociation inhibitor. The proportion of Rab in the GTP-bound state, and thus able to interact with target proteins, depends on the respective activities of guanine nucleotide exchange factors and GAPs. It is therefore important to characterize the properties of these regulators of Rab cycle. Rab3 has been implicated in the exocytosis of hormones and neurotransmitters (3Geppert M. Südhof T.C. Annu. Rev. Neurosci. 1998; 21: 75-95Crossref PubMed Scopus (223) Google Scholar, 4Darchen F. Goud B. Biochimie ( Paris ). 2000; 82: 375-384Crossref PubMed Scopus (87) Google Scholar). Increasing the amount of GTP-bound Rab3A results in a reduction of the magnitude of the secretory responses (5Holz R.W. Brondyk W.H. Senter R.A. Kuizon L. Macara I.G. J. Biol. Chem. 1994; 269: 10229-10234Abstract Full Text PDF PubMed Google Scholar, 6Johannes L. Lledo P.M. Roa M. Vincent J.D. Henry J.P. Darchen F. EMBO J. 1994; 13: 2029-2037Crossref PubMed Scopus (187) Google Scholar, 7Johannes L. Lledo P.M. Chameau P. Vincent J.D. Henry J.P. Darchen F. J. Neurochem. 1998; 71: 1127-1133Crossref PubMed Scopus (28) Google Scholar, 8Geppert M. Goda Y. Stevens C.F. Südhof T.C. Nature. 1997; 387: 810-814Crossref PubMed Scopus (347) Google Scholar). Controlling the amount of Rab3:GTP or the kinetics of the GTPase reaction might therefore be of prime importance in the physiological regulation of the exocytosis process. In yeast, several GAPs for Ypt/Rab proteins have been identified (9Strom M. Vollmer P. Tan T.J. Gallwitz D. Nature. 1993; 361: 736-739Crossref PubMed Scopus (146) Google Scholar, 10Du L.L. Collins R.N. Novick P.J. J. Biol. Chem. 1998; 273: 3253-3256Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar, 11Jones S. Richardson C.J. Litt R.J. Segev N. Mol. Biol. Cell. 1998; 9: 2819-2837Crossref PubMed Scopus (29) Google Scholar, 12Vollmer P. Will E. Scheglmann D. Strom M. Gallwitz D. Eur. J. Biochem. 1999; 260: 284-290Crossref PubMed Scopus (46) Google Scholar). In mammals, only two Rab-GAPs are currently known: GAPCenA that acts on Rab6 (13Cuif M.H. Possmayer F. Zander H. Bordes N. Jollivet F. Couedel-Courteille A. Janoueix-Lerosey I. Langsley G. Bornens M. Goud B. EMBO J. 1999; 18: 1772-1782Crossref PubMed Scopus (123) Google Scholar) and Rab3-GAP that is specific for Rab3 subfamily members (14Fukui K. Sasaki T. Imazumi K. Matsuura Y. Nakanishi H. Takai Y. J. Biol. Chem. 1997; 272: 4655-4658Abstract Full Text Full Text PDF PubMed Scopus (134) Google Scholar). GAPCenA displays some homology with yeast Rab-GAPs, but the sequence of Rab3-GAP seems to be totally different and is not related to any other known protein. Previous studies have shown that the catalytic domain of Rab3-GAP is localized in the last 400 (out of 981) amino acids and that Rab3-GAP is firmly bound to another subunit of 150 kDa, which is not required for the catalytic activity of Rab3-GAP in vitro (15Nagano F. Sasaki T. Fukui K. Asakura T. Imazumi K. Takai Y. J. Biol. Chem. 1998; 273: 24781-24785Abstract Full Text Full Text PDF PubMed Scopus (56) Google Scholar). In this study, we have further characterized the biochemical properties of recombinant full-length Rab3-GAP. We show that the binding affinity of Rab3-GAP for its substrate in solution is low and that the mechanism of action of Rab3-GAP involves a critical arginine residue and an interaction with the switch regions of Rab3. Nucleotides were purchased from Roche Molecular Biochemicals and other chemicals from Sigma. Calmodulin was from Calbiochem. The cDNA encoding KIAA0066 (Rab3-GAP), kindly provided by Dr. T. Nagase, was subcloned in frame into pRSETB (Invitrogen) using polymerase chain reaction (PCR). A two-step PCR strategy was followed to mutate different arginine residues of Rab3-GAP. The corresponding sequences were cloned in pRSETB. pET-3A-Rab3A and pGEX-2T-Rab3A-(1–217) have been described previously (16Zahraoui A. Touchot N. Chardin P. Tavitian A. J. Biol. Chem. 1989; 264: 12394-12401Abstract Full Text PDF PubMed Google Scholar, 17Johannes L. Perez F. Laran-Chich M.P. Henry J.P. Darchen F. Eur. J. Biochem. 1996; 239: 362-368Crossref PubMed Scopus (7) Google Scholar). pGEX-2T-Rabphilin-(1–206) was a kind gift of Drs S. H. Chung and R. W. Holz. To express His6-tagged Rab3A-(1–185), a stop codon was introduced by PCR into the sequence of hu-Rab3A, followed by in-frame subcloning into pET-15B (Novagen). The sequences generated by PCR were verified by dideoxy sequencing. Rab3-GAP was found to be highly toxic in Escherichia coli BL21 or DH5α. For expression, freshly transformed C41 cells (a strain kindly provided by B. Miroux and J. Walker, which derived from BL21 DE3 (18Miroux B. Walker J.E. J. Mol. Biol. 1996; 260: 289-298Crossref PubMed Scopus (1578) Google Scholar)) were grown in LB medium at 37 °C. Isopropyl-β-d-thiogalactopyranoside (1 mm) was added at A 600 ∼0.7, and the culture was grown overnight at 25 °C. Bacterial pellets were resuspended in 20 mm Tris/Cl, pH 8.0, 300 mm NaCl, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 5 μg/ml pepstatin, 0.5 mmPefabloc, 0.5 mm diisopropyl fluorophosphate and rapidly frozen in liquid nitrogen. After thawing, the cells were incubated with lysozyme (1 mg/ml) for 15 min at 4 °C and briefly sonicated. Cell extracts were centrifuged for 30 min at 100,000 × g. Ammonium sulfate (10%, w/v) was added to the supernatant, which was stirred on ice for 1 h and centrifuged at 20,000 ×g for 30 min. Ammonium sulfate (40%, final concentration) was added to the supernatant, which was stirred on ice for 1 h and spun at 20,000 × g for 30 min. The pellet was resuspended in 20 mm Tris/Cl, pH 8.0, 100 mmNaCl, aprotinin, leupeptin, pepstatin (all at 5 μg/ml), 0.5 mm Pefabloc. His6-Rab3-GAP was then purified by affinity chromatography on cobalt-agarose (CLONTECH) according to the manufacturer's instructions and dialyzed overnight against 20 mm Tris/Cl, pH 8.0, 100 mm NaCl, 0.1 mm dithiothreitol. The protein was estimated to be >80% pure by SDS-polyacrylamide gel electrophoresis, Coomassie Blue staining, and densitometry scanning using NIH image (Fig. 7). When required, the protein was concentrated in Biomax 10 centrifugal filters (Millipore). After rapid freezing in liquid nitrogen, the protein was stored at −80 °C without apparent loss of activity.Figure 7Effect of arginine substitutions on the enzymatic activity of Rab3-GAP. A, SDS-polyacrylamide gel electrophoresis of purified His6-tagged wild type and mutated Rab3-GAP proteins used in this study. Gels were stained with Coomassie Blue. B, increasing amounts of Rab3-GAP (▴, wild type (WT); ○, R619A; ♦, R700A; ●, R728A; ■, R753A) were incubated with 1 μm Rab3A-[γ-32P]GTP for 6 min at 25 °C. Data are expressed as the percentage of the radioactivity retained on nitrocellulose filters in the absence of Rab3-GAP. Each point is the mean of two experimental determinations.View Large Image Figure ViewerDownload Hi-res image Download (PPT) His6-Rab3A-(1–185) was expressed in C41 cells and purified onto cobalt-agarose (CLONTECH) using standard procedures. Rab3A-(1–220) was expressed in BL21 DE3 cells and purified as described previously (16Zahraoui A. Touchot N. Chardin P. Tavitian A. J. Biol. Chem. 1989; 264: 12394-12401Abstract Full Text PDF PubMed Google Scholar). Rab3A-(1–217) and Rabphilin-(1–206) were expressed as glutathioneS-transferase fusion proteins and purified by affinity chromatography with glutathione-Sepharose (Amersham Pharmacia Biotech) under standard conditions. Purified Rab3 proteins were dialyzed overnight against 20 mm Tris/Cl, pH 8.0, 100 mm NaCl, 1 mm MgCl2, 1 μm GDP. When required the proteins were concentrated in Biomax 10 centrifugal filters (Millipore). The concentration of Rab3 proteins was determined by [3H]GTP (7.7 Ci/mmol, Amersham Pharmacia Biotech) binding. The concentrations of other proteins were determined either by a Bradford assay (Pierce) or by the BCA assay (Bio-Rad) using bovine serum albumin (Sigma) as a standard. Rab3A was incubated for 5 min at 30 °C in 20 mm Tris/Cl, pH 8.0, 3 mm EDTA, 1 mm MgCl2, 100 mm NaCl, 0.5 mm dithiothreitol, 0.15% bovine serum albumin, 0.033 μm [γ-32P]GTP (5000 Ci/mmol, Amersham Pharmacia Biotech), and cold GTP (10-fold molar excess over Rab3). Nucleotides in excess were removed by passage through ProbeQuant G25 columns (Amersham Pharmacia Biotech). 5 mmMgCl2 were immediately added to the eluate, which was stored at 4 °C. [γ-32P]GTP-loaded Rab3A (typically 10 pmol) was then incubated with Rab3-GAP for various periods of time (typically 6 min) at 25 °C. At the end of the incubation, the mixture was diluted in 1 ml of ice-cold washing buffer (20 mm Tris/Cl, pH 8.0, 100 mm NaCl, 1 mm MgCl2), applied to a nitrocellulose filter (0.45 μm, Millipore). The filters were rinsed with 3 × 2.5 ml ice-cold washing buffer, and the radioactivity retained on the filters was determined by scintillation counting. To obtain K m and k cat values, data were analyzed by nonlinear regression with the program "Kaleidagraph" (Synergy Software) using the Michaelis-Menten equation. For the competition experiments, the model equation used wasB = B max S/(S + K d), where Bis the bound ligand, B max the number of binding sites, S the concentration of ligand, andK d the dissociation constant. Rab3A was loaded with [γ-32P]GTP, freed of excess nucleotides by passage through ProbeQuant G25 columns (Amersham Pharmacia Biotech), and incubated with Rab3-GAP (0–200 μm) in 7 μl of 20 mm Tris/Cl, pH 8.0, 100 mm NaCl, 1 mm MgCl2, 1 mm dithiothreitol. 20 pmol of GST-Rabphilin () immobilized on glutathione-Sepharose (in 5 μl) were added, and the mixture was kept on ice for 15 min. The reaction was terminated by the addition of 2.5 ml of ice-cold wash buffer (20 mm Tris/Cl, pH 8.0, 100 mm NaCl, 1 mm MgCl2) and filtered through glass fiber filters (Whatman GF/C). Rab3A was poorly retained on these filters in the absence of GST-Rabphilin and glutathione-Sepharose. The filters were washed with 3 × 2.5 ml and processed for scintillation counting. Calcium-buffered solutions were prepared according to Ref. 19Fohr K.J. Warchol W. Gratzl M. Methods Enzymol. 1993; 221: 149-157Crossref PubMed Scopus (97) Google Scholar. His6-tagged full-length Rab3-GAP (981 amino acids in length) was found to be highly toxic in E. coli. However, it was readily expressed in a soluble form in the mutant strain C41 (18Miroux B. Walker J.E. J. Mol. Biol. 1996; 260: 289-298Crossref PubMed Scopus (1578) Google Scholar). The protein was purified to near homogeneity by affinity chromatography onto cobalt-agarose. Recombinant His6-tagged Rab3-GAP was able to accelerate the hydrolysis of [γ-32P]GTP bound to Rab3A, as measured in a filter binding assay (Fig. 1). Preliminary experiments did not reveal major differences between prenylated or nonprenylated Rab3A. Therefore, nonisoprenylated Rab3A proteins expressed in E. coli were used in this study. Rab3-GAP was found to be active either on full-length Rab3A-(1–220) or on COOH-terminally truncated Rab3A proteins (Fig. 1). Most of the experiments were performed with His6-tagged Rab3A-(1–185). To determine K m, 50 nm Rab3-GAP was incubated with 1 μm [γ-32P]GTP-loaded Rab3A and increasing concentrations of GTP-loaded Rab3A, for 0–10 min at 25 °C. The initial rate of GTP hydrolysis was determined and plotted as a function of Rab3A:GTP. As illustrated in Fig.2, high amounts of Rab3:GTP were required to saturate the activity of Rab3-GAP on [γ-32P]GTP-loaded Rab3A. Values forK m (75 ± 10 μm; mean ± S.D.) and k cat (1 ± 0.3 s−1; mean ± S.D.) of Rab3-GAP were derived from four independent experiments. Since the intrinsic GTP hydrolysis rate of Rab3A is ∼3 × 10−4s−1 (data not shown), one can conclude that Rab3-GAP increases GTP hydrolysis rate by a factor of ∼3000. This is significantly less than the acceleration factor determined for Gyps, the GAPs for yeast Ypt/Rab proteins (20Albert S. Will E. Gallwitz D. EMBO J. 1999; 18: 5216-5225Crossref PubMed Scopus (130) Google Scholar), and Ras-GAPs. However, thek cat values are within the range of values reported for the different GAPs (1–19 s−1(20Albert S. Will E. Gallwitz D. EMBO J. 1999; 18: 5216-5225Crossref PubMed Scopus (130) Google Scholar, 21Gideon P. John J. Frech M. Lautwein A. Clark R. Scheffler J.E. Wittinghofer A. Mol. Cell. Biol. 1992; 12: 2050-2056Crossref PubMed Scopus (248) Google Scholar)). The lower acceleration factor observed for Rab3-GAP may be partly due to the high intrinsic GTP hydrolysis rate of Rab3A. Next, we determined the binding affinity of Rab3 to Rab3-GAP. Increasing amounts of Rab3A loaded with the nonhydrolyzable analog GTPγS were added into a standard GAP reaction (Fig.3 A). Here, the IC50 value was taken as a measure of theK d. A value of 75 ± 28 μm(mean ± S.D.) was derived from six independent experiments. This value is similar to that of K m. Accordingly, Rab3A:GTP and Rab3A:GTPγS behave similarly in competition experiments (Fig. 3 A). Rab3A Q81L was also used to determine the affinity of Rab3A for Rab3-GAP. Glutamine 81 in Rab3A corresponds to glutamine 61 in Ras that plays a critical role in the nucleophilic attack of the γ-phosphate of GTP. Accordingly, this mutated protein has no intrinsic GTPase activity. Even in the presence of large amounts (up to 300 nm) of Rab3-GAP, no GTP hydrolysis could be detected (Fig. 4 B). GTP-loaded Rab3A Q81L competes with wild type Rab3A in a GAP assay (Fig.3 B) with an IC50 value of 5.4 ± 0.4 μm (mean ± S.D., n = 3). The fact that Rab3A Q81L:GTP is a 10-fold more potent inhibitor of Rab3-GAP than GTPγS-loaded Rab3A suggests a difference in their conformational state. Such a difference has been observed previously in the case of Ras. Mutations of Gln-61, and especially Q61L, increase the affinity between p21-Ras:GTP and GAP (21Gideon P. John J. Frech M. Lautwein A. Clark R. Scheffler J.E. Wittinghofer A. Mol. Cell. Biol. 1992; 12: 2050-2056Crossref PubMed Scopus (248) Google Scholar). It was also reported that p120-GAP has a higher affinity for Ras:GDP:AlF3 than for the triphosphate state (ground state) of Ras (22Mittal R. Ahmadian M.R. Goody R.S. Wittinghofer A. Science. 1996; 273: 115-117Crossref PubMed Scopus (193) Google Scholar). Structural and biochemical studies led to the conclusion that the ternary complex formed by Ras:GDP, Ras-GAP, and AlF3 is a transition state analog (23Scheffzek K. Ahmadian M.R. Kabsch W. Wiesmuller L. Lautwein A. Schmitz F. Wittinghofer A. Science. 1997; 277: 333-338Crossref PubMed Scopus (1202) Google Scholar, 24Scheffzek K. Ahmadian M.R. Wittinghofer A. Trends Biochem. Sci. 1998; 23: 257-262Abstract Full Text Full Text PDF PubMed Scopus (355) Google Scholar). We tested the ability of Rab3:GDP to interact with Rab3-GAP in the presence of aluminum fluoride. As shown in Fig.3 B, Rab3A:GDP:AlFx is a potent inhibitor of the activity of Rab3-GAP on Rab3A:GTP. The IC50 value determined in these experiments was 15 ± 9 μm(mean ± S.D., n = 3). It seems likely that this value, which is very similar to that determined with Rab3A Q81L, is a measure of the affinity of Rab3A, in the transition state, for Rab3-GAP. GTP hydrolysis is responsible for the disruption of the interaction of G proteins with their effectors. We investigated whether Rab3-GAP was able to act on Rab3A complexed with an effector molecule. Rabphilin is one of the putative effectors of Rab3A (4Darchen F. Goud B. Biochimie ( Paris ). 2000; 82: 375-384Crossref PubMed Scopus (87) Google Scholar). As illustrated in Fig. 4 A, the Rab3-binding domain of Rabphilin inhibited Rab3-GAP activity. This indicates that Rab3-GAP- and Rabphilin-binding domains overlap. Rab3A interacts with Rabphilin via its switch I region, which encompasses the so-called effector domain, and via other regions, termed Rab-complementary determining regions, which contact a conserved SGAWFF motif in Rabphilin (25Ostermeier C. Brunger A.T. Cell. 1999; 96: 363-374Abstract Full Text Full Text PDF PubMed Scopus (291) Google Scholar). In agreement with the ability of Rabphilin to compete with Rab3-GAP, the mutation V55E in the switch I region of Rab3A was found to disrupt the interaction with Rab3-GAP (Fig. 4,B and C). Another putative effector of Rab3 is calmodulin. In the presence of calcium ions, calmodulin (CaM) solubilizes isoprenylated membrane-bound Rab3 (26Park J.B. Farnsworth C.C. Glomset J.A. J. Biol. Chem. 1997; 272: 20857-20865Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar). Furthermore, binding of Ca2+/CaM to Rab3 correlates with the effect of Rab3A on the secretory activity of endocrine cells (27Coppola T. Perret-Menoud V. Lüthi S. Farnsworth C.C. Glomset J.A. Regazzi R. EMBO J. 1999; 18: 5885-5891Crossref PubMed Scopus (83) Google Scholar). The addition of Ca2+/CaM in the filter assay did not change the activity of Rab3-GAP (Fig.5 A). K m is not affected by the presence of Ca2+/CaM (not shown). Furthermore, Ca2+/CaM did not interfere with the binding of Rab3-GAP to Rab3A, as demonstrated by the fact that the inhibitory effect of GTP-loaded Rab3A Q81L on the activity of Rab3-GAP was not significantly modified by the presence of Ca2+/CaM (Fig.5 B). In the absence of CaM, we did not observe any effect of Ca2+ ions neither on the activity of Rab3-GAP nor on its interaction with Rab3 (Fig. 5 B). The mechanism of GAP action on several GTPases, including Ras and Rho, has been elucidated recently. One important feature of this mechanism is the existence of a critical arginine residue, which is positioned in the active site, binds to the γ-phosphate group and neutralizes the developing charge during the reaction (24Scheffzek K. Ahmadian M.R. Wittinghofer A. Trends Biochem. Sci. 1998; 23: 257-262Abstract Full Text Full Text PDF PubMed Scopus (355) Google Scholar). In Gα subunits, an arginine residue provided by the GTPase itself plays a similar role. It seemed, therefore, possible that Rab-GAPs also use an "arginine finger" to accelerate the GTPase reaction. To identify putative catalytic arginine residues in Rab3-GAP, sequence homology searching was performed. As reported previously (14Fukui K. Sasaki T. Imazumi K. Matsuura Y. Nakanishi H. Takai Y. J. Biol. Chem. 1997; 272: 4655-4658Abstract Full Text Full Text PDF PubMed Scopus (134) Google Scholar), no significant homology was found with any known protein, including the GAPs for other Rab proteins. However, several uncharacterized sequences were found in sequence data banks with similarity to human Rab3-GAP (Fig. 6). Within this group of sequences, several arginine residues appeared to be conserved. Arginines 619, 700, 728, and 753 belonging to the catalytic domain of Rab3-GAP defined by Fukui et al. (14Fukui K. Sasaki T. Imazumi K. Matsuura Y. Nakanishi H. Takai Y. J. Biol. Chem. 1997; 272: 4655-4658Abstract Full Text Full Text PDF PubMed Scopus (134) Google Scholar) were mutated in alanine. The corresponding proteins were expressed in E. coli, purified, and tested in the filter assay. Rab3-GAP R619A, R700A, and R753A had a normal activity, but Rab3-GAP R728A was found to be inactive on Rab3A (Fig.7). To ascertain that the reduced activity of Rab3-GAP R728A was not due to improper folding, its interaction with Rab3A was assayed. First, Rab3-GAP R728A was found to compete with wild type Rab3-GAP (Fig.8 A). Second, Rab3-GAP R728A was able to inhibit the binding of GTP-loaded Rab3A to GST-Rabphilin (Fig. 8 B). In these experiments (Fig. 8, A andB), IC50 values were in good agreement with the affinity of Rab3A for Rab3-GAP. At high concentrations, Rab3-GAP R728A had very low but detectable GAP activity (Fig. 8 B). However, this activity was too weak to account for the diminished signal in the Rab3A/Rabphilin binding assay (i.e. the [γ-32P]GTP bound to Rab3A and retained on the filters). The binding of Rab3-GAP to Rab3A Q81L was also assayed. In sharp contrast to wild type Rab3-GAP, the mutated protein did not interact with the GTPase-deficient Rab3A mutant (Fig.8 C). Ras-GAPs and Rho-GAPs act by ordering the switch regions of the GTP-binding protein such that the catalytic glutamine residue is positioned properly and by supplying an arginine residue to the active site (23Scheffzek K. Ahmadian M.R. Kabsch W. Wiesmuller L. Lautwein A. Schmitz F. Wittinghofer A. Science. 1997; 277: 333-338Crossref PubMed Scopus (1202) Google Scholar, 28Rittinger K. Walker P.A. Eccleston J.F. Smerdon S.J. Gamblin S.J. Nature. 1997; 389: 758-762Crossref PubMed Scopus (355) Google Scholar). ARF-GAP only stabilizes switch II, but coatomer (an effector of ARF) might provide catalytic residues, since it contributes a further ∼1000-fold acceleration of the reaction rate (29Goldberg J. Cell. 1999; 96: 893-902Abstract Full Text Full Text PDF PubMed Scopus (248) Google Scholar). Gα subunits possess an arginine finger very similar to that of Ras-GAP, which is responsible for the higher intrinsic rate of GTP hydrolysis, compared with small GTP-binding proteins (30Coleman D.E. Berghuis A.M. Lee E. Linder M.E. Gilman A.G. Sprang S.R. Science. 1994; 265: 1405-1412Crossref PubMed Scopus (753) Google Scholar, 31Sondek J. Lambright D.G. Noel J.P. Hamm H.E. Sigler P.B. Nature. 1994; 372: 276-279Crossref PubMed Scopus (535) Google Scholar). RGS proteins are GAPs for Gα GTPases, which act by "stabilizing" the switch regions (32Tesmer J.J. Berman D.M. Gilman A.G. Sprang S.R. Cell. 1997; 89: 251-261Abstract Full Text Full Text PDF PubMed Scopus (687) Google Scholar, 33Srinivasa S.P. Watson N. Overton M.C. Blumer K.J. J. Biol. Chem. 1998; 273: 1529-1533Abstract Full Text Full Text PDF PubMed Scopus (106) Google Scholar). Therefore, the mechanism responsible for the acceleration of GTP hydrolysis seems to be universal, although the work is shared out differently in each family of GTPases. Our results suggest that Rab3-GAP is functionally related to Ras-GAPs and Rho-GAPs. First, the overlap between Rab3-GAP and Rabphilin-binding sites on Rab3A and the insensitivity of Rab3A V55E to Rab3-GAP argue for an interaction between GAP and the Rab3A switch regions. The higher affinity of Rab3-GAP for the transition state, as mimicked by Rab3:GDP:AlFx, than for the ground state (represented by Rab3:GTPγS) further suggests that Rab3-GAP stabilizes the transition state conformation of the switch regions. Second, the lack of catalytic activity of Rab3-GAP R728A is consistent with Rab3-GAP introducing an arginine finger into the catalytic site. Since Rab3-GAP R728A interacts normally with wild type Rab3A, its loss of function cannot be accounted for by disruption of binding to the substrate. Interestingly, it was found that the mutation R728A disrupted the interaction of Rab3-GAP with the GTPase-deficient Rab3A Q81L protein. This observation might indicate that Arg-728 is positioned, within the Rab3-Rab3-GAP complex, in the vicinity of Gln-81 and, therefore, near the catalytic site. During completion of this work, Albertet al. (20Albert S. Will E. Gallwitz D. EMBO J. 1999; 18: 5216-5225Crossref PubMed Scopus (130) Google Scholar) reported the identification of arginine residues essential for the activity of Gyp1 and Gyp7, two yeast Ypt/Rab-GAPs. Arg-458 in Gyp7 contributes a factor of ∼1000 to the total 2 × 105-fold stimulation of the GTPase reaction. Another arginine residue, Arg-615, accelerates the GTPase reaction by ∼30-fold. Arg-615 might be analogous to Arg-903 in p120Ras-GAP or Lys-122 in p50Rho-GAP, which play a role in stabilizing the finger loop. Altogether, these observations suggest that Rab-GAPs also use an arginine finger mechanism to accelerate the reaction. However, the crystal structure of GppNHp-bound Rab3A (35Dumas J.J. Zhu Z. Connolly J.L. Lambright D.G. Structure ( Lond. ). 1999; 7: 413-423Abstract Full Text Full Text PDF PubMed Scopus (102) Google Scholar) revealed that the γ-phosphate is buried in Rab3A, in contrast to Ras. The side chains of Phe-51 and Ser-31 seem to be detrimental to the insertion of an arginine residue into the catalytic site. Future structural studies of the Rab3-Rab3-GAP complex will have to determine whether Rab3-GAP can open the conformation of the catalytic site to introduce a catalytic residue or whether it accelerates GTP hydrolysis by reorienting intrinsic residues (Ser-31, Ser-53, and Gln-81) in a manner similar to the RGS proteins. Rab3-GAP and Rabphilin binding to Rab3A are mutually exclusive. This finding, which confirms previous observations made with a brain extract enriched in Rab3-GAP activity (36Kishida S. Shirataki H. Sasaki T. Kato M. Kaibuchi K. Takai Y. J. Biol. Chem. 1993; 268: 22259-22261Abstract Full Text PDF PubMed Google Scholar), raises the question of the mechanisms that control the duration of the GTP signal. Due to the overlap between the binding domain of Rab3-GAP and of the effector, Rab3-GAP would be able to modulate the kinetics of GTP hydrolysis and the duration of the GTP signal only if the lifetime of the Rab3-effector complex were short. In the case of Ras, the lifetime of Ras-Raf1 complex was shown to be short (37Sydor J.R. Engelhard M. Wittinghofer A. Goody R.S. Herrmann C. Biochemistry. 1998; 37: 14292-14299Crossref PubMed Scopus (109) Google Scholar). Hence, Ras-GAP may control the duration of the "on" state and thus the number of Raf1 molecules activated by Ras. In the case of ARF, the scenario differs. ARF:GTP forms a stoichiometric and probably stable complex with coatomer. However, ARF-GAP can interact with this complex and thus controls its duration (29Goldberg J. Cell. 1999; 96: 893-902Abstract Full Text Full Text PDF PubMed Scopus (248) Google Scholar). 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Thus, different mechanisms are used to drive the translocation of GAPs toward membrane compartments. Oishi et al. (43Oishi H. Sasaki T. Nagano F. Ikeda W. Ohya T. Wada M. Ide N. Nakanishi H. Takai Y. J. Biol. Chem. 1998; 273: 34580-34585Abstract Full Text Full Text PDF PubMed Scopus (49) Google Scholar) reported that Rab3-GAP was enriched in the synaptic soluble fraction. Although the vast majority of Rab3-GAP was found in the cytosol, some immunoreactivity was found in the membrane fractions. Furthermore, the immunostaining of Rab3-GAP in primary cultured hippocampal neurons was not diffuse but enriched at synaptic contacts. It is noteworthy that Burstein et al. (34Burstein E.S. Linko-Stentz K. Lu Z.J. Macara I.G. J. Biol. Chem. 1991; 266: 2689-2692Abstract Full Text PDF PubMed Google Scholar) found that a Rab3-GAP activity from rat brain was equally distributed between cytosolic and membrane fractions. Recruitment of Rab3-GAP on membranes could thus account for the targeting of Rab3-GAP to nerve terminals and, most importantly, could facilitate the interaction with its substrates by increasing its local concentration. An attractive possibility is that the subcellular localization of Rab3-GAP is dependent on the noncatalytic p150 subunit. In agreement with Oishi et al. (43Oishi H. Sasaki T. Nagano F. Ikeda W. Ohya T. Wada M. Ide N. Nakanishi H. Takai Y. J. Biol. Chem. 1998; 273: 34580-34585Abstract Full Text Full Text PDF PubMed Scopus (49) Google Scholar), we also observed that overexpression of wild type Rab3-GAP in PC12 cells had no detectable effect on the secretory activity (not shown). Thus, the amount of cellular Rab3-GAP is not limiting. Other factors probably control the activity of Rab3-GAP. Under the conditions used in this study (i.e. in the absence of phospholipids and of isoprenylation of Rab3A), neither calcium ions nor calmodulin had any detectable effect on the activity of Rab3-GAP. Since a number of studies have highlighted the role of GTP hydrolysis by Rab3 in the secretory process, it will be of prime importance to characterize the parameters (like membrane recruitment) that control the activity of Rab3-GAP. We thank Dr. T. Nagase for the kind gift of the plasmid encoding KIAA 0066 (Rab3-GAP), Drs. R. Holz and S. H. Chung for the vector encoding GST-Rabphilin1–206, Dr. J. Walker and B. Miroux for the E. coli strain C41, and Dr. M. Chabre for helpful discussion and critical reading of the manuscript.