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In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells

2008; National Academy of Sciences; Volume: 105; Issue: 52 Linguagem: Inglês

10.1073/pnas.0809680106

1091-6490

Nathaniel S. Hwang, Shyni Varghese, H. Janice Lee, Zijun Zhang, Zhaohui Ye, Jongwoo Bae, Linzhao Cheng, Jennifer H. Elisseeff,

3D Printing in Biomedical Research

Development of clinically relevant regenerative medicine therapies using human embryonic stem cells (hESCs) requires production of a simple and readily expandable cell population that can be directed to form functional 3D tissue in an in vivo environment. We describe an efficient derivation method and characterization of mesenchymal stem cells (MSCs) from hESCs (hESCd-MSCs) that have multilineage differentiation potential and are capable of producing fat, cartilage, and bone in vitro. Furthermore, we highlight their in vivo survival and commitment to the chondrogenic lineage in a microenvironment comprising chondrocyte-secreted morphogenetic factors and hydrogels. Normal cartilage architecture was established in rat osteochondral defects after treatment with chondrogenically-committed hESCd-MSCs. In view of the limited available cell sources for tissue engineering applications, these embryonic-derived cells show significant potential in musculoskeletal tissue regeneration applications.