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Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA

1981; Oxford University Press; Volume: 9; Issue: 15 Linguagem: Inglês

10.1093/nar/9.15.3647

1362-4962

R. Bruce Wallace, Monica Schöld, Merrie Jo Johnson, Piotr Dembek, Keiichi Itakura,

CRISPR and Genetic Engineering

A nonadecanucleotide has been used both as a site specific mutagen to introduce an T→-A transversion mutation in the human β-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specificity of the oligonucleotide as a mutagen and as a hybridization probe provide a general method for producing site specific mutations in DNA cloned in plasmid vectors such as pBR322.