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Polydimethylsiloxane microfluidic chip with integrated microheater and thermal sensor

2009; American Institute of Physics; Volume: 3; Issue: 1 Linguagem: Inglês

10.1063/1.3058587

1932-1058

Jinbo Wu, Wenbin Cao, Weijia Wen, Donald C. Chang, Ping Sheng,

Analytical Chemistry and Sensors

A microheater and a thermal sensor were fabricated inside elastomeric polydimethylsiloxane microchannels by injecting silver paint (or other conductive materials) into the channels. With a high-precision control scheme, microheaters can be used for rapid heating, with precise temperature control and uniform thermal distribution. Using such a microheater and feedback system, a polymerase chain reaction experiment was carried out whereas the DNA was successfully amplified in 25 cycles, with 1 min per cycle.