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Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes

2009; Wiley; Volume: 108; Issue: 5 Linguagem: Inglês

10.1002/jcb.22344

1097-4644

Adriano Spreafico, Federico Chellini, Bruno Frediani, Giulia Bernardini, S. Niccolini, Tommaso Serchi, Giulia Collodel, Alessandro Paffetti, Vittorio Fossombroni, Mauro Galeazzi, R. Marcolongo, Annalisa Santucci,

Osteoarthritis Treatment and Mechanisms

Abstract The aim of the present study was to demonstrate the mitogenic and differentiating properties of platelet‐rich plasma releasates (PRPr) on human chondrocytes in mono‐ and three‐dimensional cultures. In order to assess if PRPr supplementation could maintain the chondrocyte phenotype or at least inhibit the cell de‐differentiation even after several days in culture, we performed a proteomic study on several cell cultures independently grown, for different periods of time, in culture medium with FCS, human serum (HS), and releasates obtained from PRP and platelet‐poor plasma (PPP). We found that PRP treatment actually induced in chondrocytes the expression of proteins (some of which novel) involved in differentiation. J. Cell. Biochem. 108: 1153–1165, 2009. © 2009 Wiley‐Liss, Inc.