Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers
2000; Wiley; Volume: 79; Issue: 1 Linguagem: Inglês
10.1159/000031201
ISSN1423-0410
AutoresE.H. Kostelijk, Claudia C. Folman, C.W.N. Gouwerok, Christine Krämer, A. J. Verhoeven, Dirk de Korte,
Tópico(s)Blood groups and transfusion
ResumoVox SanguinisVolume 79, Issue 1 p. 21-26 Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers E.H. Kostelijk, E.H. Kostelijk CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorC.C. Folman, C.C. Folman CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorC.W.N. Gouwerok, C.W.N. Gouwerok CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorC.M. Kramer, C.M. Kramer CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorA.J. Verhoeven, A.J. Verhoeven CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorD. de Korte, D. de Korte CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this author E.H. Kostelijk, E.H. Kostelijk CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorC.C. Folman, C.C. Folman CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorC.W.N. Gouwerok, C.W.N. Gouwerok CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorC.M. Kramer, C.M. Kramer CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorA.J. Verhoeven, A.J. Verhoeven CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this authorD. de Korte, D. de Korte CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The NetherlandsSearch for more papers by this author First published: 27 March 2003 https://doi.org/10.1046/j.1423-0410.2000.7910021.xCitations: 25 Dr. D. de Korte, CLB/Sanquin, Plesmanlaan 125, 1066CX Amsterdam (The Netherlands), Tel.+31 20 5123317, Fax +31 20 5123310, E-Mail [email protected] AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Background and Objectives: Glycocalicin (GC) is a proteolytic fragment of Gplb and can conveniently be measured in supernatants of platelet concentrates (PCs) by means of a sandwich ELISA. Because of the convenience of the assay and easy sample storage, we tested its suitability as a sensitive platelet activation parameter during PC storage. Material and Methods: Filtered PCs in plasma or additive solution were made from 5 pooled buffy coats and were subsequently stored during 8 days at 22±2°C. Correlation coefficients (r) were calculated after comparison of GC levels with platelet parameters. Results: A significant increase in GC concentration was found on all subsequent sampling days. PC stored in plasma showed GC levels that correlated well with the soluble P-selectin levels (r = 0.7506), P-selectin (CD62P) expression on platelet membranes (r = 0.8843), morphology scores according to Kunicki (r =−0.7102), lactate concentrations (r = 0.9216), glucose concentrations (r =−0.8913) and β-thromboglobulin (β-TG) concentrations (r = 0.8913). In PCs stored in additive solution, the correlation coefficients with these markers were 0.9209 with soluble P-selectin, 0.7161 with CD62P expression, −0.7474 with morphology score, −0.8908 with glucose concentrations, 0.8923 with lactate concentrations and 0.8908 with β-TG concentrations. Conclusions: The GC concentration correlates well with sensitive platelet (activation) parameters, rendering it a sensitive and convenient parameter for platelet activation. References 1 Coller BS, Kalomiris E, Steinberg M, Scudder LE: Evidence that glycocalicin circulates in normal plasma. 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