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Phenotypic and molecular analysis of ph 1 ‐chromosome‐positive acute lymphoblastic leukemia cell lines

1993; Wiley; Volume: 53; Issue: 3 Linguagem: Inglês

10.1002/ijc.2910530318

1097-0215

Takuya Miyagi, Junko H. Ohyashiki, Kenji Yamato, H. Phillip Koeffler, Ichiro Miyoshi,

T-cell and Retrovirus Studies

Abstract We have established 2 Philadelphia chromosome (Ph 1 )‐positive acute lymphoblastic leukemia (ALL) cell lines, designated PALL‐1 and PALL‐2, from distinct adult Ph 1 ‐positive ALL patients. PALL‐1 was established in nude mice, and PALL‐2 was established in culture. Both retained the Ph 1 chromosome and expressed the ALL type bcr / abl chimeric mRNA containing the junction of the first exon of BCR gene (eI) and second exon of c‐ abl gene (a2). PALL‐1 and PALL‐2 expressed CD34 surface antigen which is characteristic of early hematopoietic progenitor cells. PALL‐2 expressed antigens for both pre‐B and early myeloid cells and had rearrangements of both the heavy chain of immunoglobulin gene and the β chain of T‐cell‐receptor gene. Both PALL‐1 and PALL‐2 expressed detectable levels of p 53 gene RNA. Polymerase‐chain‐reaction‐single‐strand conformation polymorphism (PCR‐SSCP) analysis of the p 53 gene showed a normal pattern of mobility in both cell lines. Taken together, the 2 cell lines had features of Ph 1 ‐positive ALL (i) hematopoietic progenitor cells with pre‐B‐cell phenotype and, (ii) activation of el‐a2 type bcr / abl oncogene without alterations of p 53 gene. These unique lines should provide a valuable tool for studying the pathogenesis of Ph 1 ‐positive ALL.