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Cytological studies of early stages of powdery mildew in barley and wheat. VII. Reciprocal translocation of a fluorescent dye between barley coleoptile cells and conidia

1981; Academic Press; Volume: 18; Issue: 2 Linguagem: Inglês

10.1016/s0048-4059(81)80042-2

1876-7974

Hitoshi Kunoh, Hiroshi Ishizaki,

Powdery Mildew Fungal Diseases

This study examines the translocation of a fluorescent dye from barley coleoptile cells to conidia and related structures of Erysiphe graminis hordei and vice versa during infection using micromanipulation. Conidia were first incubated on coleoptiles floating over CaCl2 solution containing acridine orange. Soon after the emergence of appressorial germ tubes, selected conidia with a primary germ tube and appressorial germ tube were transferred by a microneedle to coleoptiles floating on a CaCl2 solution containing no acridine orange. The transferred conidia were further incubated and observed with a fluorescence microscope at various times during incubation. Acridine orange was absorbed by conidia before transfer and subsequently was translocated to appressoriaj leached from appressorial apices and finally infiltrated into papillae and regions of the host cell walls corresponding to halos. The results suggest that molecular translocation occurs between host and pathogen prior to haustorial formation.