Preparation and Evaluation of Inactivated Venezuelan Equine Encephalitis Vaccines
2010; Wiley; Volume: 19; Issue: 6 Linguagem: Inglês
10.1111/j.1439-0450.1972.tb00430.x
ISSN0931-2021
AutoresM. Mussgay, G. H. Bergold, E. Weiland, S Ueberschär,
Tópico(s)Viral Infections and Vectors
ResumoZentralblatt für Veterinärmedizin Reihe BVolume 19, Issue 6 p. 511-517 Preparation and Evaluation of Inactivated Venezuelan Equine Encephalitis Vaccines Prof. Dr. M. Mussgay, Corresponding Author Prof. Dr. M. Mussgay Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this authorDr. G. H. Bergold, Corresponding Author Dr. G. H. Bergold Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this authorDr. Emilie Weiland, Corresponding Author Dr. Emilie Weiland Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this authorDr. S. Ueberschär, Corresponding Author Dr. S. Ueberschär Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this author Prof. Dr. M. Mussgay, Corresponding Author Prof. Dr. M. Mussgay Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this authorDr. G. H. Bergold, Corresponding Author Dr. G. H. Bergold Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this authorDr. Emilie Weiland, Corresponding Author Dr. Emilie Weiland Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this authorDr. S. Ueberschär, Corresponding Author Dr. S. Ueberschär Federal Research Institute for Animal Virus Diseases, Tübingen, Postfach 1149, BRD (West-Germany) and the Centro de Microbiología y Biología Celular, Laboratorio Virus de Animales, Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Caracas, Venezuela Bundesforschungsanstalt für Viruskrankheiten der Tiere, Postfach 1149, 74 Tübingen, BRD (West-Germany). Instituto Venezolano de Investigaciones Cientificas (I. V. I. C.), Apartado 1827, Caracas, Venezuela.Search for more papers by this author First published: August 1972 https://doi.org/10.1111/j.1439-0450.1972.tb00430.xCitations: 3AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Summary Potency of inactivated VEE vaccines prepared with the attenuated TC 83 strain and the virulent Goajira strain was compared in mice. With both strains, vaccines with the highest amount of median protective dose (PD50) units were obtained by treatment of formalin-inactivated virus with Tween 80 and tri(n-butyl)phosphate (TNBP) and saponin; they were followed by formalin-inactivated saponin-treated vaccines. Vaccines obtained by treatment of formalin-inactivated virus with Tween 80-TNBP were still superior to vaccines containing formalin-inactivated virus without additional treatment. Gross changes of the structure of the virus particles in each of the vaccines could not be observed in the electron microscope. Résumé Préparation et évaluation de vaccins inactivés contre la encéphalite équine vénézuélienne L'efficacité de vaccins inactivés en utilisant les souches TC 83 (attenuée) et Goajira (virulente) du virus de la encéphalomyélite équine vénézuélienne a été comparé dans le souris. Avec les deux souches des vaccins montrant un nombre maximal d'unités moyennes protectives (PD50) ont été obtenus par le traitement du virus inactivé par formaline avec tween 80-TNBP et saponine; ils étaient suivis par les vaccins formalinisés et traités avec saponine. Les vaccins obtenus par un traitement du virus formalinisé avec tween 80-TNBP étaient toujours superieurs aux préparations qui contenaient le virus inactivé par formaline sans traitement additionel. Aucune modification morphologique des virions dans chaqu'un des vaccins n'a pas été observé au microscope électronique. Resumen Preparación y evaluación de vacunas inactivadas contra la encefalitis equina venezolana Se comparó en ratones la eficacia de diversas vacunas inactivadas elaboradas con las estirpes TC 83 (atenuada) y Goajira (virulenta) del virus de la encefalomielitis equina venezolana. En ambas estirpes contenían las vacunas, obtenidas mediante tratamiento del virus formalinizado con tween 80-TNBF y saponina, la cantidad máxima de unidades protectoras medias; en cuanto a eficacia, le seguían las vacunas formalizadas tratadas con saponina. Las vacunas obtenidas por un tratamiento del virus formalinizado con tween 80-TNBF eran aún superiores a las preparaciones que contenían virus inactivado por el formol sin tratamiento adicional. No se ha observado con el microscopio electrónico ninguna modificación morfológica en la estructura de las partículas virales de estas vacunas. Zusammenfassung Herstellung und Prüfung von inaktivierten Vakzinen der venezolanischen Pferdeencephalitis Die Wirksamkeit von inaktivierten Vakzinen, welche aus Material des attenuierten TC 83-Stammes und des virulenten Goajira-Stammes des Virus der venezolanischen Pferdeencephalitis hergestellt worden waren, wurde in Mäusen verglichen. Bei beiden Stämmen enthielten die durch Behandlung von formolinaktiviertem Virus mit Tween 80-Tri(n-butyl)phosphat (TNBP) und Saponin gewonnenen Vakzinen die größte Anzahl von PD50-Einheiten (median protective dosis); in der Wirksamkeit folgten diesen Vakzinen die Impfstoffe mit formolinaktiviertem, saponinbehandeltem Virus. Vakzinen, die formolinaktiviertes, Tween 80-TNBP behandeltes Virus enthielten, waren immer noch den Vakzinen überlegen, die durch Inaktivierung des Virus mit Formalin ohne zusätzliche Behandlung hergestellt worden waren. In keiner der Vakzinen konnten elektronenoptisch auffallende Veränderungen der Morphologie der Virusteilchen beobachtet werden. References 1 Alevizatos, A. C., R. W. McKinney and R. D. Feigin, 1967: Live, attenuated Venezuelan equine encephalomyelitis virus vaccine. I. Clinical effects in man. Amer. J. Trop. Med. Hyg. 16, 762–768. 10.4269/ajtmh.1967.16.762 CASPubMedWeb of Science®Google Scholar 2 Bergold, G. H., and R. 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