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Ketolide Resistance Conferred by Short Peptides

1998; Elsevier BV; Volume: 273; Issue: 32 Linguagem: Inglês

10.1074/jbc.273.32.20073

1083-351X

Shaila Tripathi, Patricia Kloss, Alexander S. Mankin,

Antibiotic Resistance in Bacteria

Clones expressing pentapeptides conferring resistance to a ketolide antibiotic, HMR3004, were selected from a random pentapeptide mini-gene library. The pentapeptide MRFFV conferred the highest level of resistance and was encoded in three different mini-genes. Comparison of amino acid sequences of peptides conferring resistance to a ketolide with those conferring resistance to erythromycin reveals a correspondence between the peptide sequence and the chemical structure of macrolide antibiotic, indicating possible interaction between the peptide and the drug on the ribosome. Based on these observations, a “bottle brush” model of action of macrolide resistance peptides is proposed, in which newly translated peptide interacts with the macrolide molecule on the ribosome and actively displaces it from its binding site. Temporal “cleaning” of the ribosome from the bound antibiotic may be sufficient to allow continuation of protein synthesis even despite the presence of the drug in the medium. Clones expressing pentapeptides conferring resistance to a ketolide antibiotic, HMR3004, were selected from a random pentapeptide mini-gene library. The pentapeptide MRFFV conferred the highest level of resistance and was encoded in three different mini-genes. Comparison of amino acid sequences of peptides conferring resistance to a ketolide with those conferring resistance to erythromycin reveals a correspondence between the peptide sequence and the chemical structure of macrolide antibiotic, indicating possible interaction between the peptide and the drug on the ribosome. Based on these observations, a “bottle brush” model of action of macrolide resistance peptides is proposed, in which newly translated peptide interacts with the macrolide molecule on the ribosome and actively displaces it from its binding site. Temporal “cleaning” of the ribosome from the bound antibiotic may be sufficient to allow continuation of protein synthesis even despite the presence of the drug in the medium. Erythromycin and other macrolides are important antibacterial antibiotics. The primary mode of action of macrolides is inhibition of protein synthesis, although they can also interfere with ribosome assembly (1Oleinick N.L. Corcoran J.W. Hahn F.E. Antibiotics III: Mechanism of Action of Antimicrobial and Antitumor Agents. Springer-Verlag, New York1975: 396-419Google Scholar, 2Vazquez D. Corcoran J.W. Hahn F.E. Antibiotics III: Mechanism of Action of Antimicrobial and Antitumor Agents. Springer-Verlag, New York1975: 459-479Google Scholar, 3Chittum H.S. Champney W.S. Curr. Microbiol. 1995; 30: 273-279Crossref PubMed Scopus (67) Google Scholar). The best studied macrolide, erythromycin, binds to the large ribosomal subunit in the vicinity of the peptidyl transferase center, where it forms tight contacts with rRNA (4Sigmund C.D. Morgan E.A. Proc. Natl. Acad. Sci. U. S. A. 1982; 79: 5602-5606Crossref PubMed Scopus (39) Google Scholar, 5Moazed D. Noller H.F. Biochimie ( Paris ). 1987; 69: 879-884Crossref PubMed Scopus (287) Google Scholar, 6Weisblum B. Antimicrob. Agents Chemother. 1995; 39: 577-585Crossref PubMed Scopus (845) Google Scholar) and maybe ribosomal proteins (7Teraoka H. Nierhaus K.H. J. Mol. Biol. 1978; 126: 185-193Crossref PubMed Scopus (52) Google Scholar, 8Chittum H.S. Champney W.S. J. Bacteriol. 1994; 176: 6192-6198Crossref PubMed Scopus (117) Google Scholar). Although the molecular mechanisms of action of erythromycin remain obscure, it is clear that the antibiotic blocks the elongation step of translation during early rounds of protein synthesis. The drug has a high affinity for ribosomes with nascent peptides shorter than 2–5 amino acids, but does not bind to ribosomes that carry long nascent peptide chains (9Cundliffe E. Gale F.R.S. Cundliffe E. Reynolds P.E. Richmond M.H. Waring M.J. The Molecular Basis of Antibiotic Action. John Wiley & Sons, London1972: 278-379Google Scholar). It was suggested that erythromycin sterically hinders growth of the nascent peptide chain (2Vazquez D. Corcoran J.W. Hahn F.E. Antibiotics III: Mechanism of Action of Antimicrobial and Antitumor Agents. Springer-Verlag, New York1975: 459-479Google Scholar) and may promote dissociation of peptidyl-tRNA (10Menninger J.R. Coleman R.A. Tsai L.-N. Mol. Gen. Genet. 1994; 243: 225-233Crossref PubMed Scopus (34) Google Scholar, 11Menninger J.R. Otto D.P. Antimicrob. Agents Chemother. 1982; 21: 810-818Crossref Scopus (124) Google Scholar). The clinical use of macrolides is significantly hampered by the growing number of resistant strains. Different mechanisms of resistance have been described (reviewed in Ref. 6Weisblum B. Antimicrob. Agents Chemother. 1995; 39: 577-585Crossref PubMed Scopus (845) Google Scholar). We have recently described a novel mechanism of erythromycin resistance, which is based on interaction of specific short peptides with the ribosome. The discovery of this resistance mechanism came from an observation that overexpression of a short segment of Escherichia coli 23 S rRNA (positions 1235–1268) rendered cells resistant to erythromycin (12Tenson T. DeBlasio A. Mankin A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 5641-5646Crossref PubMed Scopus (84) Google Scholar, 13Tenson T. Mankin A. Biochem. Cell Biol. 1995; 73: 1061-1070Crossref PubMed Scopus (19) Google Scholar). Mutational and biochemical analyses demonstrated that resistance is caused by translation of a pentapeptide open reading frame (ORF) 1The abbreviations used are: ORFopen reading frameE-peptidepentapeptide conferring erythromycin resistanceK-peptidepentapeptide conferring ketolide resistanceIPTGisopropyl-1-thio-β-d-galactopyranoside. encoded in E. coli 23 S rRNA and is mediated by interaction of the newly translated pentapeptide with the ribosome. The rRNA-encoded pentapeptide is not normally expressed because the Shine-Dalgarno region of the peptide ORF is sequestered in the 23 S rRNA secondary structure. However, the peptide expression can be activated by site-specific fragmentation of rRNA or by rRNA mutations that increase accessibility of the Shine-Dalgarno region of E-peptide ORF (14Dam M. Douthwaite S. Tenson T. Mankin A.S. J. Mol. Biol. 1996; 259: 1-6Crossref PubMed Scopus (32) Google Scholar). In order to get insights into peptide structural features that are essential for erythromycin resistance, other erythromycin resistance peptides were selected from in vivo expressed random peptide libraries (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). It was found that only short peptides, ranging in size from 3 to 6 amino acids, with specific amino acid sequence can confer erythromycin resistance. Analysis of more than 70 pentapeptides that can confer resistance to erythromycin (E-peptides) revealed a consensus sequence, MXLXV, which could be recognized in the majority of E-peptides and was especially pronounced in the most active E-peptides that could confer very high levels of erythromycin resistance (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). open reading frame pentapeptide conferring erythromycin resistance pentapeptide conferring ketolide resistance isopropyl-1-thio-β-d-galactopyranoside. In vitro studies suggested that the ribosome is the most plausible target of action of E-peptides (12Tenson T. DeBlasio A. Mankin A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 5641-5646Crossref PubMed Scopus (84) Google Scholar). Interestingly, we observed that chemically synthesized peptides added exogenously to the cell-free translation mixture did not render ribosomes resistant to erythromycin. In contrast, ribosomes that could translate E-peptide mRNA exhibited increased resistance to erythromycin in a cell-free translation system (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). Based on this observation, a model ofcis-acting E-peptide was proposed where a newly synthesized E-peptide remains tightly bound to the ribosome and prevents binding of erythromycin into its functional site. Although this model accounted for most of the experimental data, it did not satisfactorily explain why synthetic peptides were not functional in vitro. Furthermore, in order to explain erythromycin resistance of cells expressing E-peptide, we had to invoke a remote possibility that the ribosome-bound E-peptide could be displaced by a newly initiated growing nascent peptide chain. According to this model, E-peptide did not have to interact directly with the drug, and the only requirement for peptide activity was its tight binding to the ribosome in the vicinity of the erythromycin binding site. To test whether there is an interaction between the drug and peptide on the ribosome, we selected peptides conferring resistance to a chemically different macrolide with a mode of action similar to that of erythromycin. We report here isolation of pentapeptides conferring resistance to a new class of macrolide antibiotics, ketolides. The results of this study show a correspondence between peptide structure and the chemical nature of macrolides to which peptide confers resistance, suggesting direct interaction between the resistance peptide and macrolide antibiotic on the ribosome. This finding allowed us to propose a novel model of action of macrolide resistance peptides. Ketolide (HMR3004) was from Roussel Uclaf, France. Erythromycin was purchased from Sigma. E. coli JM109 strain (16Yanisch-Perron C. Viera J. Messing J. Gene ( Amst. ). 1985; 33: 103-119Crossref PubMed Scopus (11480) Google Scholar) (endA1, recA1,gyrA96, thi, hsdR17 (rK−, mK+ relA1, supE44, D(lac-proAB), F', traD36,proAB, lacIqZDM15) was used for propagation of the random pentapeptide library. Construction of the library in the pPOT1AE vector has been described previously (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar), and the physical map of the library plasmid is shown in Fig. 1. The mini-gene library (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar) was transformed into competent E. coli JM109 cells and plated onto agar plates containing 100 μg/ml ampicillin, 60 μm ketolide, and 2 mm IPTG. Plates were incubated overnight at 37 °C. Twenty-five individual colonies that appeared on the plate were grown in liquid cultures. Plasmids were isolated from 3-ml cultures and used to transform fresh E. coli cells. Transformants were plated onto ampicillin plates, and individual colonies of transformed cells were streaked onto plates containing 100 μg/ml ampicillin and 60 μm ketolide with or without addition of 2 mm IPTG. All the selected clones exhibited IPTG-dependent ketolide-resistant phenotype co-transferable with the plasmids. The level of ketolide resistance of the isolated clones was tested in liquid cultures as described previously (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). Mini-genes in the selected plasmids were sequenced using CCW sequencing primer d(GCCATCGGAAGCTGTGG) (Fig. 1). Construction of a random five-codon mini-gene library was described previously (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). In this library, mini-genes, containing the ATG initiator codon, followed by four random codons and the TAA terminator codon, were cloned into the pPOT1AE vector where they are expressed from an IPTG-inducible Ptac promoter (Fig.1). This library has been used previously to select for clones that express peptides rendering cells resistant to erythromycin (E-peptides) (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). In the present study, we asked the question whether the amino acid sequence of the selected resistance peptides depends on the chemical structure of macrolide antibiotic used in selection. Ketolides represent a new generation of 14-member-ring macrolide antibiotics exhibiting increased activity toward erythromycin-resistant ribosomes (17Agouridas C. Bonnefoy A. Chantot J.F. Antimicrob. Agents Chemother. 1997; 41: 2149-2158Crossref PubMed Google Scholar, 18Ednie L.M. Spangler S.K. Jacobs M.R. Appelbaum P.C. Antimicrob. Agents Chemother. 1997; 41: 1033-1036Crossref PubMed Google Scholar). The l-cladinose sugar moiety at position 3 of the erythromycin macrolide ring is replaced by a keto group in ketolides (Fig. 2). The general mechanism of action of ketolides is probably similar to that of erythromycin, and both macrolides have overlapping binding sites on the ribosome. 2L. Xiong, S. Shah, P. Mauvais, and A. S. Mankin, unpublished data., 3S. Douthwaite, personal communication.We used one of the ketolide derivatives, HMR3004, to select clones expressing ketolide resistance peptides. The minimal inhibitory concentration of HMR3004 for E. coliJM109 was 10 μm. Clones expressing ketolide resistance peptides (K-peptides) were selected by plating the library onto an agar plate containing 2 mm IPTG and 60 μmketolide. Several clones appeared on the plate after 24 h of incubation, and 25 clones were analyzed. Resistance phenotypes were co-transferable with plasmids isolated from the clones. Furthermore, dependence of ketolide resistance on the presence of IPTG clearly indicated that it was mediated by expression of plasmid-encoded peptide mini-genes (Fig. 3). Sequencing of plasmids from 25 isolated ketolide-resistant clones revealed 6 different peptide mini-genes (Table I), each found in several independent isolates. Remarkably, three mini-genes (clones K3, K9, and K17), which had different nucleotide sequences, encoded the same pentapeptide, MRFFV. The minimal inhibitory concentration of HMR3004 for clones expressing different K-peptides ranged from 60 to 100 μm and thus, significantly exceeded the drug concentration required to inhibit growth of the controlE. coli cells (Table II).Table IThe amino acid sequences of the selected K-peptides and nucleotide sequences of corresponding mini-genesCloneNo. of isolatesMini-gene sequencePeptide sequenceK17ATG TTA TAT AAA CCT TAAM L Y K PK23ATG AAG GAT ACT TCA TAAM K D T SK155ATG TCG TGG AAA ATA TAAM S W K IK33ATG CGC TTT TTT GTC TAAM R F F VK94ATG CGG TTC TTT GTT TAAM R F F VK173ATG CGG TTT TTT GTT TAAM R F F VThe number of independent isolates containing the same mini-gene is shown in the second column. Open table in a new tab Table IIMinimal inhibitory concentration of ketolide HMR3004 for E. coli JM109 cells transformed with an empty vector pPOT1AE or recombinant plasmids expressing K-peptidesClonePeptide sequenceMICaMIC, minimal inhibitory concentration.μmpPOT1AENone10K1M L Y K P80K2M K D T S60K3M R F F V100K15M S W K I80The antibiotic MIC was determined in liquid cultures as described in Ref. 15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar.a MIC, minimal inhibitory concentration. Open table in a new tab The number of independent isolates containing the same mini-gene is shown in the second column. The antibiotic MIC was determined in liquid cultures as described in Ref. 15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar. In previous studies, we found that the amino acid sequence of E-peptides conferring erythromycin resistance conformed to the consensus MXLXV, where the second and the fourth positions were significantly less conserved than the third and the fifth (the first position was occupied by methionine by default) (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). Interestingly, the sequence of the K-peptide MRFFV (see Table I) closely resembled that of the E-peptides MRLFV, which conferred resistance to a very high erythromycin concentration (up to 1 mg/ml) (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). We tested whether peptides selected with one antibiotic could confer resistance to the other (K-peptides to erythromycin and, conversely, E-peptides to ketolide). The clone K3 expressing the MRFFV K-peptide and the clone expressing the E-peptide MRLFV were streaked onto agar plates containing ampicillin, IPTG, and either ketolide or erythromycin (Fig. 4). Cells expressing E-peptide were resistant to low concentrations of ketolide, while K-peptide could confer resistance to low concentrations of erythromycin (data not shown). Remarkably, however, only E-peptide rendered cells resistant to high erythromycin concentration and, conversely, only K-peptide conferred high level of ketolide resistance. Thus, different peptides exhibit a clear specificity toward different types of macrolide antibiotics. Unraveling the mechanism of peptide-mediated macrolide resistance may provide important clues for understanding the mode of action of macrolide antibiotics. It may also provide insights into interactions between the ribosome and the nascent peptide. In the current study, we investigated whether resistance peptides act simply by competing with the antibiotic for the ribosomal binding site, or whether macrolide resistance conferred by short peptides is mediated by direct interaction between the peptide and the drug on the ribosome. To address this question, peptides conferring resistance to a ketolide, a macrolide antibiotic different from erythromycin, were selected and their amino acid sequences were compared with those of E-peptides. The previously selected erythromycin resistance peptides conformed to a general sequence consensus, MXLXV, where the third position was the most conserved and occupied by leucine in the majority of E-peptides (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). Among the ketolide resistance peptides selected in the current study, neither had leucine in the third position. This result indicates that different peptides confer resistance to different macrolides. This conclusion was corroborated by comparing the resistance patterns conferred by a very similar E-peptide and K-peptide (MRLFV and MRFFV, respectively). The E-peptide rendered cells resistant to high concentrations of erythromycin, but not ketolide; conversely, expression of K-peptide afforded high level of ketolide resistance but did not protect the cell from high erythromycin concentrations (Fig. 4). Thus, the nature of the expressed peptide determines to which macrolide the cell will become resistant. Out of 25 analyzed ketolide-resistant clones, 10 expressed K-peptide MRFFV. This peptide is apparently one of the “best” peptides conferring resistance to the ketolide HMR3004 used in the selection. Cells expressing this peptide exhibited the highest level of ketolide resistance compared with other selected clones (Table II). Furthermore, this same peptide was encoded in three different mini-genes (Table I), demonstrating strong selection for a specific amino acid sequence. Since one of the best E-peptides had the sequence MRLFV, which differed from the K-peptide MRFFV only in the third amino acid position, one can suppose that, within this context, it is the third amino acid (leucine, in the E-peptide or phenylalanine, in K-peptide) that allows the peptide to discriminate between the two macrolides. We have previously demonstrated the cis-mode of E-peptide action so that only the ribosome that translated an E-peptide became resistant to erythromycin, while exogenously added E-peptide did not affect inhibition of cell-free translation by erythromycin (12Tenson T. DeBlasio A. Mankin A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 5641-5646Crossref PubMed Scopus (84) Google Scholar). Based on this observation, a model of action of E-peptides was proposed in which the newly translated E-peptide remained bound to the ribosome in the vicinity of the peptidyl transferase center thus blocking the erythromycin-binding site (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). Within this model, the peptide did not interact directly with the erythromycin molecule and resistance depended only on the relative affinities of the E-peptide and the drug to the ribosome. Our new results, however, show that the macrolide and newly synthesized peptide do interact with each other; such interaction is reflected in correlation between the chemical structure of macrolide and the amino acid sequence of resistance peptides. To reconcile the model with the new experimental data, we suggest that the newly synthesized peptide does not just passively occupy the drug binding site, but instead, actively displaces the macrolide from the ribosome (Fig. 5). During this process, the peptide directly interacts with the drug. In the previous model, which was based on an idea of tight association of E-peptides with the ribosome, we had to explain why E-peptide bound in the nascent peptide channel does not inhibit protein synthesis. Therefore, we had to speculate that after initiation of a new polypeptide synthesis, the growing nascent peptide chain has either to displace the E-peptide from its binding site or go around it. This problem is easily solved within the new model which does not require association of the resistance peptide with the ribosome. Instead, the peptide acts as a “bottle brush” that “cleans” the ribosome from the bound antibiotic. After antibiotic is removed, the ribosome can either initiate synthesis of a new polypeptide or bind another molecule of the drug. In the former case, when the nascent peptide becomes longer than 5 amino acids, the ribosome will become “resistant” to macrolides until completion of polypeptide translation because macrolides cannot bind to ribosomes with long nascent peptide chains (19Pestka S. Antimicrob. Agents Chemother. 1974; 5: 255-267Crossref PubMed Scopus (33) Google Scholar, 20Otaka T. Kaji A. Proc. Natl. Acad. Sci. U. S. A. 1975; 72: 2649-2652Crossref PubMed Scopus (49) Google Scholar). Thus, translation of macrolide resistance peptides opens for ribosomes a window of opportunity to translated cellular proteins. Notably, the new model is fully compatible with the cis-mode of the peptide action, because only the ribosome that translated the resistance peptide mini-gene would become transiently competent for translation of other genes, which, in an experiment, would be observed as antibiotic resistance. The correspondence between the peptide amino acid sequence and chemical nature of the macrolide strongly suggests a possibility of interaction between the resistance peptide and the antibiotic. However, free peptide apparently does not interact with the drug, since even 1000-fold excess of the synthetic E-peptide added to the cell-free translation system did not reduce inhibitory action of erythromycin (12Tenson T. DeBlasio A. Mankin A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 5641-5646Crossref PubMed Scopus (84) Google Scholar). Therefore, the peptide-drug interaction occurs most probably only on the ribosome. Previously, we have shown that only short peptides (3–6 amino acids long) could confer resistance to erythromycin (15Tenson T. Xiong L. Kloss P. Mankin A.S. J. Biol. Chem. 1997; 272: 17425-17430Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar). The size of active peptides is compatible with direct interaction between resistance peptide and the drug on the ribosome. Erythromycin hinders growth of nascent polypeptide when it reaches the size of 2–5 amino acids. Therefore, when the ribosome decodes the stop codon of an E- or K-peptide mini-gene, the newly synthesized pentapeptide should be in contact with the ribosome-bound antibiotic. The ribosome may constrain the newly synthesized peptide in a conformation competent for specific interaction with the antibiotic. Such interaction may reduce affinity of the drug to its binding site on the ribosome, and it can be ejected together with the completed peptide. The known mechanisms of antibiotic resistance are usually divided into three main groups: 1) mechanisms affecting accumulation of the drug in the cell, 2) mechanisms involving modification of the drug target, and, finally, 3) mechanisms based on modification of the drug. The proposed mechanism of action of macrolide resistance peptides does not fall within any of the three categories, but rather represents a hybrid of the latter two groups, since “modification of the drug” (its ability to bind to the ribosome) occurs directly within the target (the ribosome). Multiple examples of peptide sequences capable of conferring resistance to different macrolide antibiotics suggest that similar mechanisms of antibiotic resistance may potentially operate in nature and may account for macrolide resistance of some clinical pathogens. We thank Dr. P. Mauvais (Roussel Uclaf, Romainville, France) for encouragement and Dr. S. Douthwaite (University of Odense, Odense, Denmark) for communicating unpublished results.