A microchip electrophoresis system with integrated in-plane electrodes for contactless conductivity detection
2002; Wiley; Volume: 23; Issue: 21 Linguagem: Inglês
10.1002/1522-2683(200211)23
ISSN1522-2683
AutoresJan Lichtenberg, Ν. F. de Rooij, Elisabeth Verpoorte,
Tópico(s)Analytical Chemistry and Sensors
ResumoELECTROPHORESISVolume 23, Issue 21 p. 3769-3780 Research Article A microchip electrophoresis system with integrated in-plane electrodes for contactless conductivity detection Jan Lichtenberg, Jan Lichtenberg Institute of Microtechnology, University of Neuchâtel, Neuchâtel, SwitzerlandSearch for more papers by this authorNico F. de Rooij, Nico F. de Rooij Institute of Microtechnology, University of Neuchâtel, Neuchâtel, SwitzerlandSearch for more papers by this authorElisabeth Verpoorte, Corresponding Author Elisabeth Verpoorte [email protected] Institute of Microtechnology, University of Neuchâtel, Neuchâtel, SwitzerlandInstitute of Microtechnology, University of Neuchâtel, Rue Jaquet-Droz 1, CH-2007 Neuchâtel, Switzerland Fax: +41-32-720-57-11===Search for more papers by this author Jan Lichtenberg, Jan Lichtenberg Institute of Microtechnology, University of Neuchâtel, Neuchâtel, SwitzerlandSearch for more papers by this authorNico F. de Rooij, Nico F. de Rooij Institute of Microtechnology, University of Neuchâtel, Neuchâtel, SwitzerlandSearch for more papers by this authorElisabeth Verpoorte, Corresponding Author Elisabeth Verpoorte [email protected] Institute of Microtechnology, University of Neuchâtel, Neuchâtel, SwitzerlandInstitute of Microtechnology, University of Neuchâtel, Rue Jaquet-Droz 1, CH-2007 Neuchâtel, Switzerland Fax: +41-32-720-57-11===Search for more papers by this author First published: 12 November 2002 https://doi.org/10.1002/1522-2683(200211)23:21 3.0.CO;2-ECitations: 115AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract We present a new approach for contactless conductivity detection for microchip-based capillary electrophoresis (CE). The detector integrates easily with well-known microfabrication techniques for glass-based microfluidic devices. Platinum electrodes are structured in recesses in-plane with the microchannel network after glass etching, which allows precise positioning and batch fabrication of the electrodes. A thin glass wall of 10–15 νm separates the electrodes and the buffer electrolyte in the separation channel to achieve the electrical insulation necessary for contactless operation. The effective separation length is 34 mm, with a channel width of 50 νm and depth of 12 νm. Microchip CE devices with conductivity detection were characterized in terms of sensitivity and linearity of response, and were tested using samples containing up to three small cations. 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