Multiple relationships between rate of oxidative phosphorylation and Δ µ̃ H in rat liver mitochondria
1985; Wiley; Volume: 193; Issue: 2 Linguagem: Inglês
10.1016/0014-5793(85)80168-x
ISSN1873-3468
AutoresMario Zoratti, Valeria Petronilli,
Tópico(s)ATP Synthase and ATPases Research
ResumoThe relationship between rate of ATP synthesis and transmembrane electrochemical proton gradient has been determined in rat liver mitochondria oxidizing succinate, using the respiratory inhibitor malonate or the uncoupler FCCP to decrease Δ µ̃ H progressively. As previously reported [(1982) Eur. J. Biochem. 126, 443‐451] two different relationships are obtained depending on the method used. Evidence is presented that this result is not due to underestimation of the Δ µ̃ H maintained by fast‐respiring mitochondria, as recently suggested [(1985) FEBS Lett. 181, 323‐327].
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