Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley
1994; NRC Research Press; Volume: 37; Issue: 4 Linguagem: Inglês
10.1139/g94-078
ISSN1480-3321
AutoresА. В. Сорокін, Frank Marthe, Andreas Houben, Uta Pich, Andreas Graner, G. Künzel,
Tópico(s)Wheat and Barley Genetics and Pathology
ResumoA new strategy has been devised and used for the physical localization of genetically mapped restriction fragment length polymorphism (RFLP) clones to barley chromosomes. Morphologically distinct translocation chromosomes from synchronized root-tip meristems were microisolated and their DNA was used as a template for polymerase chain reaction with sequence-specific primers. Four RFLP clones were assigned to cytologically defined segments of chromosome 5. This related approximately one-third of the map length of linkage group 5 to approximately one-fifth of the mitotic metaphase length of chromosome 5. The technique may substantially contribute to the connection of the RFLP-based genetic linkage maps with cytological markers of the barley chromosomes.Key words: Hordeum vulgare, microisolated chromosomes, translocation, polymerase chain reaction, physical RFLP mapping.
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