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BIBTEX RIS

Combinatorial alanine-scanning

2001; Elsevier BV; Volume: 5; Issue: 3 Linguagem: Inglês

10.1016/s1367-5931(00)00206-4

ISSN

1879-0402

Autores

Kim Morrison, Gregory A. Weiss,

Tópico(s)

Chemical Synthesis and Analysis

Resumo

Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine substitution examines the contribution of an individual amino acid sidechain to the functionality of the protein. The recently described method of shotgun scanning uses phage-displayed libraries of alanine-substituted proteins for high-throughput analysis.

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