Interaction between Radiation and Drug Damage in Mammalian Cells: I. Delayed Expression of Actinomycin D/X-Ray Effects in Exponential and Plateau Phase Cells
1975; Radiation Research Society; Volume: 63; Issue: 2 Linguagem: Inglês
10.2307/3574159
ISSN1938-5404
AutoresAnthony J. Piro, Catherine C. Taylor, James A. Belli,
Tópico(s)Cancer therapeutics and mechanisms
ResumoThe survival characteristics of V79 Chinese hamster cells exposed to either X-radiation or actinomycin-D and subsequently treated with the other modality were studied. Exponentially growing cells were exposed to either 820 R, 50 kVp X rays or 3.0 μg/ml AMD for 30 min and the survivors were exposed to graded doses of the other agent 2, 3, and 7 days afterward. The previously irradiated cells did not differ in their response to subsequent AMD, whereas the cells which had survived AMD demonstrated a decrease in extrapolation number and $\tilde{D}_{Q}$ when exposed to X rays as compared to cells not previously treated with AMD. The X-ray two-dose response was studied in cells exposed to 3.0 μg/ml 3 days before. The AMD treated cells had a lower maximum recovery ratio than controls and subsequent survival with time between doses was lower than in controls. Similar experiments were done with stationary (plateau phase) V79 cells. The response of stationary cells to AMD after either similar d...
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