Determination of amino acids as phenyl thiohydantoin derivatives III. Quantitative determination of 3-phenyl-2-thiohydantoins from paper chromatograms
1960; Elsevier BV; Volume: 41; Issue: 1 Linguagem: Inglês
10.1016/0006-3002(60)90364-4
ISSN1878-2434
Autores Tópico(s)Synthesis and Reactivity of Sulfur-Containing Compounds
ResumoA method for quantitative determination of the 3-phenyl-2-thiohydantoin derivatives (PTH) from the naturally occuring amino acids is described. The PTH's are synthesized by a micro synthetic procedure from protein hydrolysates (0.5-1 mg protein) and separated on paper chromatograms. The spots are located with the aid of fluorescent screen and eluted from the paper with ethanol. The quantitative calculations are based on the optical density at 269 and 320 mμ of the ethanolic eluates.
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