Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica
2005; Elsevier BV; Volume: 36; Issue: 5-6 Linguagem: Inglês
10.1016/j.enzmictec.2005.01.011
ISSN1879-0909
AutoresSyed Imteyaz Alam, Smita Dube, G. S. N. Reddy, B. K. Bhattacharya, S. Shivaji, Lokendra Singh,
Tópico(s)Protein Hydrolysis and Bioactive Peptides
ResumoOne anaerobic, proteolytic bacterium isolated from Schirmacher oasis, Antarctica was characterised taxonomically. Based on morphology, biochemical characteristics and 16S rRNA sequence the isolate was identified as Clostridium species with closest similarity with Clostridium subterminale. Isolate was psychrotrophic forming maximum cell mass between 5 and 10 °C and produced extracellular protease. Growth was observed in the pH range of 6.5–8.5 with optimum at pH 8. Protease was purified 12.7-fold with a total yield of 26.2%. Effect of temperature, pH and salt concentration on enzyme activity were studied. Protease was found to be a serine-type metaloenzyme, which is active in a broad range of pH. It was moderately thermolabile and resistant to SDS. Enzyme kinetics revealed a tendency to decrease Km with increase in temperature for casein substrate.
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